LOCUS       Exported               10530 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pAPn, complete sequence.
ACCESSION   KR997564
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10530)
  AUTHORS   Zare B, Malboobi MA, Sabet MS, Norouzi P.
  TITLE     High plant transformation efficiency using a new chimeric P-DNA 
            derived from sugar beet
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10530)
  AUTHORS   Zare B, Malboobi MA, Sabet MS, Norouzi P.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2015) Plant Biotechnology, NIGEB, Pajoohesh Blvd.,
            Tehran 009821, Iran
REFERENCE   3  (bases 1 to 10530)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10530
                     /organism="Binary vector pAPn"
                     /mol_type="other DNA"
                     /db_xref="taxon:1795585"
     misc_feature    1..7998
                     /label=backbone of pART27 binary vector
                     /note="backbone of pART27 binary vector"
     oriT            140..249
                     /note="incP origin of transfer"
     mobile_element  309..1076
                     /mobile_element_type="insertion sequence:IS1"
                     /note="prokaryotic transposable element"
     CDS             1701..2849
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(4209..4797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             5894..6682
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7357..7887
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      7365..7978
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     misc_feature    7999..8307
                     /label=PB, a new chimeric P-DNA derived from sugar beet
                     /note="PB, a new chimeric P-DNA derived from sugar beet"
     misc_feature    7999..8112
                     /label=ACR region of pTiC58
                     /note="ACR region of pTiC58"
     regulatory      8308..8729
                     /regulatory_class="promoter"
                     /gene="nos"
     gene            8308..8729
                     /gene="nos"
                     /label=nos
                     /note="nopaline synthase"
     promoter        complement(8347..8365)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8372..8388)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8546..8729
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             8730..9551
                     /codon_start=1
                     /product="neomycin phosphotransferase"
                     /function="kanamycin resistance"
                     /label=neomycin phosphotransferase
                     /note="kanamycin resistance protein"
                     /protein_id="AMD82484.1"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      9552..10393
                     /regulatory_class="terminator"
                     /gene="nos"
     gene            9552..10393
                     /gene="nos"
                     /label=nos
                     /note="nopaline synthase"
     misc_feature    10394..10487
                     /label=PB, a new chimeric P-DNA derived from sugar beet
                     /note="PB, a new chimeric P-DNA derived from sugar beet"
     misc_feature    10488..10530
                     /label=C-clusters region of pTiC58
                     /note="C-clusters region of pTiC58"