LOCUS       Exported                5350 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pAP3neo DNA, complete sequence.
ACCESSION   AB003468
VERSION     .
KEYWORDS    Amp; beta-lactamase; neomycin phosphotransferase; neo.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Kobori M, Ikeda Y, Nara H, Kato M, Kumegawa M, Nojima H, Kawashima 
            H.
  TITLE     Large scale isolation of osteoclast-specific genes by an improved 
            method involving the preparation of a subtracted cDNA library
  JOURNAL   Genes Cells 3 (7), 459-475 (1998)
  PUBMED    9753427
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Nojima H.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-1997) Hiroshi Nojima, Research Institute for 
            Microbial Diseases, Osaka University, Department of Molecular 
            Genetics; Yamadaoka 3-1, Suita, Osaka 565-0871, Japan 
            (E-mail:snj-0212@biken.osaka-u.ac.jp, Tel:81-6-6875-3980, 
            Fax:81-6-6875-5192)
REFERENCE   3  (bases 1 to 5350)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Cloning vector pAP3neo"
                     /mol_type="other DNA"
                     /db_xref="taxon:69403"
     polyA_signal    43..177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      47..268
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late polyadenylation signal"
     misc_feature    428..568
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      572..1452
                     /label=ColE1-derived plasmid replication origin
                     /note="ColE1-derived plasmid replication origin"
     rep_origin      complement(754..1342)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1513..2373)
                     /codon_start=1
                     /gene="Amp"
                     /product="beta-lactamase"
                     /label=Amp
                     /protein_id="BAA23652.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(1513..2373)
                     /gene="Amp"
                     /label=Amp
     promoter        complement(2374..2478)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2504..2959
                     /label=phage f1 region
                     /note="phage f1 region"
     primer_bind     3101..3117
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3156..3485
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      3290..3485
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      3336..3471
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3596..4390
                     /codon_start=1
                     /gene="neo"
                     /product="neomycin phosphotransferase"
                     /label=neo
                     /protein_id="BAA23653.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            3596..4390
                     /gene="neo"
                     /label=neo
     polyA_signal    4442..4576
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      4446..4638
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late polyadenylation signal"
     promoter        4654..4983
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      4788..4983
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      4834..4969
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5022..5118
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     regulatory      5188..5207
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     promoter        5188..5206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5302..5321
                     /regulatory_class="promoter"
                     /note="T3 promoter"
     promoter        complement(5303..5321)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"