LOCUS       Exported                4094 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pANY6, complete sequence.
ACCESSION   MH385156
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   An Y, Gao H, Qi X, Hart DJ, Gao S, Wang H, Xu S, Zhang Y, Liu X, Liu
            Y.
  TITLE     Three novel Escherichia coli vectors for convenient and efficient 
            molecular biological manipulations
  JOURNAL   J. Agric. Food Chem. (2018) In press
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   An Y, Gao H, Qi X, Hart DJ, Gao S, Wang H, Xu S, Zhang Y, Liu X, Liu
            Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2018) College of Biosciences and Biotechnology, 
            Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, 
            Liaoning 110161, P. R. China.
REFERENCE   3  (bases 1 to 4094)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Cloning vector pANY6"
                     /mol_type="other DNA"
                     /label=derived from Escherichia coli
                     /note="derived from Escherichia coli"
                     /db_xref="taxon:2212682"
     regulatory      37..656
                     /regulatory_class="other"
                     /note="replicon"
     rep_origin      53..641
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      712..734
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     promoter        718..736
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             768..790
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    798..1784
                     /label=ELP tag
                     /note="ELP tag"
     misc_feature    1785..2294
                     /label=intein
                     /note="intein"
     regulatory      2295..2326
                     /regulatory_class="other"
                     /note="multiple cloning sites (MCS)-1"
     CDS             2657..2962
                     /codon_start=1
                     /product="CcdB"
                     /label=CcdB
                     /protein_id="AWP40279.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     regulatory      2989..3026
                     /regulatory_class="other"
                     /note="multiple cloning sites (MCS)-2"
     terminator      3054..3101
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      3055..3101
                     /regulatory_class="terminator"
                     /note="T7 terminator"
     promoter        complement(3169..3197)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             complement(3210..4025)
                     /codon_start=1
                     /product="KanA"
                     /label=KanA
                     /protein_id="AWP40280.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPVPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"