LOCUS       Exported                3734 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pANY3, complete sequence.
ACCESSION   MH385155
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   An Y, Gao H, Qi X, Hart DJ, Gao S, Wang H, Xu S, Zhang Y, Liu X, Liu
            Y.
  TITLE     Three novel Escherichia coli vectors for convenient and efficient 
            molecular biological manipulations
  JOURNAL   J. Agric. Food Chem. (2018) In press
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   An Y, Gao H, Qi X, Hart DJ, Gao S, Wang H, Xu S, Zhang Y, Liu X, Liu
            Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2018) College of Biosciences and Biotechnology, 
            Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, 
            Liaoning 110161, P. R. China.
REFERENCE   3  (bases 1 to 3734)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Cloning vector pANY3"
                     /mol_type="other DNA"
                     /label=derived from Escherichia coli
                     /note="derived from Escherichia coli"
                     /db_xref="taxon:2212681"
     misc_feature    1..21
                     /label=His-tag
                     /note="His-tag"
     CDS             1..18
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      80..127
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      81..127
                     /regulatory_class="terminator"
                     /note="Terminator"
     promoter        complement(195..223)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             complement(236..1051)
                     /codon_start=1
                     /gene="kanA"
                     /product="KanA"
                     /label=kanA
                     /protein_id="AWP40277.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(236..1051)
                     /gene="kanA"
                     /label=kanA
     rep_origin      1173..1761
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      1284..1784
                     /regulatory_class="other"
                     /note="pMB1-replicon"
     CDS             complement(1842..2555)
                     /codon_start=1
                     /gene="clts857"
                     /product="clts857"
                     /label=clts857
                     /note="clts857 protein"
                     /protein_id="AWP40278.1"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     gene            complement(1842..2555)
                     /gene="clts857"
                     /label=clts857
     regulatory      2603..2630
                     /regulatory_class="promoter"
                     /note="pR promoter"
     RBS             2644..2652
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3 end of the 16S rRNA (Chen et al., 1994)"
     regulatory      2817..2845
                     /regulatory_class="promoter"
                     /note="pL promoter"
     regulatory      2985..3024
                     /regulatory_class="other"
                     /note="multiple cloning sites (MCS)-1"
     CDS             3354..3659
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB"
                     /label=ccdB
                     /note="ccdB toxic protein"
                     /protein_id="AWP40276.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            3354..3659
                     /gene="ccdB"
                     /label=ccdB
     regulatory      3689..3734
                     /regulatory_class="other"
                     /note="multiple cloning sites (MCS)-2"