LOCUS       Exported                8058 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pANT1200, complete sequence.
ACCESSION   AY072038
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   DeSanti CL, Strohl WR.
  TITLE     Characterization of the Streptomyces sp. strain C5 snp locus and 
            development of snp-derived expression vectors
  JOURNAL   Appl. Environ. Microbiol. 69 (3), 1647-1654 (2003)
  PUBMED    12620855
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   DeSanti CL, Strohl WR.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-DEC-2001) School of Dentistry, Case Western Reserve 
            University, 2123 Abington Rd, Cleveland, OH 44106, USA
REFERENCE   3  (bases 1 to 8058)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Expression vector pANT1200"
                     /mol_type="genomic DNA"
                     /label=used for expression in Streptomyces lividans
                     /note="used for expression in Streptomyces lividans"
                     /db_xref="taxon:184069"
     promoter        407..511
                     /gene="bla"
                     /label=AmpR promoter
     CDS             512..1372
                     /codon_start=1
                     /product="beta lactamase Bla"
                     /label=beta lactamase Bla
                     /protein_id="AAL61982.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1543..2131
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2134..2265
                     /label=ColE1
                     /note="ColE1"
     protein_bind    2419..2440
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2455..2485
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2493..2509
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2517..2533
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2690..4060)
                     /codon_start=1
                     /product="replication protein pIJ101 Rep"
                     /label=replication protein pIJ101 Rep
                     /protein_id="AAL61983.1"
                     /translation="MDPASGVIVAQTAAGTSVVLGLMRCGRIWLCPVCAATIRHKRAEE
                     ITAAVVEWIKRGGTAYLVTFTARHGHTDRLADLMDALQGTRKTPDSPRRPGAYQRLITG
                     GTWAGRRAKDGHRAADREGIRDRIGYVGMIRATEVTVGQINGWHPHIHAIVLVGGRTEG
                     ERSAKQIVATFEPTGAALDEWQGHWRSVWTAALRKVNPAFTPDDRHGVDFKRLETERDA
                     NDLAEYIAKTQDGKAPALELARADLKTATGGNVAPFELLGRIGDLTGGMTEDDAAGVGS
                     LEWNLSRWHEYERATRGRRAIEWTRYLRQMLGLDGGDTEADDLDLLLAADADGGELRAG
                     VAVTEDGWHAVTRRALDLEATRAAEGKDGNEDPAAVGERVREVLALADAADTVVVLTAG
                     EVAEAYADMLAALAQRREEATARRRREQDDDQDDDADDRQERAARHIARLASGPTSH"
     CDS             5326..6135
                     /codon_start=1
                     /product="thiostrepton resistance protein TsrR"
                     /label=thiostrepton resistance protein TsrR
                     /protein_id="AAL61984.1"
                     /translation="MTELDTIANPSDPAVQRIIDVTKPSRSNIKTTLIEDVEPLMHSIA
                     AGVEFIEVYGSDSSPFPSELLDLCGRQNIPVRLIDSSIVNQLFKGERKAKTFGIARVPR
                     PARFGDIASRRGDVVVLDGVKIVGNIGAIVRTSLALGASGIILVDSDITSIADRRLQRA
                     SRGYVFSLPVVLSGREEAIAFIRDSGMQLMTLKADGDISVKELGDNPDRLALLFGSEKG
                     GPSDLFEEASSASVSIPMMSQTESLNVSVSLGIALHERIDRNLAANR"
     misc_feature    6562..6672
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      complement(6692..6732)
                     /regulatory_class="promoter"
                     /note="snpA promoter"
     CDS             6950..7891
                     /codon_start=1
                     /product="LysR-like transcriptional activator SnpR"
                     /label=LysR-like transcriptional activator SnpR
                     /protein_id="AAL61985.1"
                     /translation="MELEVRHLRALCAIADTGSLHRAARQLGVTQPSLSTQLRRIEHEL
                     GGALFVRARTGCRPTPLGRLVLSRARPLVAELRSLVSEARAAAVGGRQLRVGSTASRAL
                     AGWLRRLRRHWQEPTLHMDVSANALLRMVADGHLDVAFVHEVEGSPLRVPEGLRVRVLV
                     QREPQFVCLPADHPAAAKPSYASPTWPTTRWMIDPTVDGEWDAVRRVLRAEGLDPRILH
                     GDYHTAASLVATGEVVTVVQPTSPSRAETAVRRLHGDPLGVRLLLAARTDTELEGVYPD
                     LAEAYGEVARQAPAYREWLERSGSGALVPALP"