LOCUS       Exported                5011 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Integrative expression vector pAnFP, complete sequence.
ACCESSION   KF706368
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Song DH, Li J, Hu XX, Xi B, Yang GL.
  TITLE     Integrative expression vector pAnFP, complete sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Song DH, Li J, Hu XX, Xi B, Yang GL.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2013) Dept.of Marine Science, College of Marine 
            Science & Engineering, Tianjin University of Science & Technology, 
            No. 29, 13th Street, TEDA, Tianjin 300457, P.R. China
REFERENCE   3  (bases 1 to 5011)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Integrative expression vector pAnFP"
                     /mol_type="other DNA"
                     /country="China"
                     /db_xref="taxon:1436143"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    707..1221
                     /note="F-up flanking region; derived from Anabaena sp. 
                     PCC7120"
     regulatory      1224..1388
                     /regulatory_class="promoter"
                     /note="light inducible psbA1 promoter derived from Anabaena
                     sp. PCC7120"
     CDS             1501..2316
                     /codon_start=1
                     /gene="nptII"
                     /product="neomycin phosphotransferase"
                     /EC_number="2.7.1.95"
                     /label=nptII
                     /note="provides kanamycin or neomycin resistance"
                     /protein_id="AHC32038.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            1501..2316
                     /gene="nptII"
                     /label=nptII
     misc_feature    2321..2783
                     /note="F-dw flanking region; derived from Anabaena sp. 
                     PCC7120"
     promoter        complement(2826..2844)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2865..2881)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2889..2905
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2913..2943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2958..2979
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3267..3855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4026..4886)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4887..4991)
                     /gene="bla"
                     /label=AmpR promoter