LOCUS       Exported                4897 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  Cloning vector pAMS749-12, complete sequence.
ACCESSION   EU056924
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Flannagan SE, Clewell DB, Sedgley CM.
  TITLE     A retrocidal plasmid in Enterococcus faecalis: passage and 
            protection
  JOURNAL   Plasmid 59 (3), 217-230 (2008)
  PUBMED    18295881
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Flannagan SE, Clewell DB, Sedgley CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2007) School of Dentistry, The University of 
            Michigan, 1011 N. University Ave., Ann Arbor, MI 48109, USA
REFERENCE   3  (bases 1 to 4897)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Cloning vector pAMS749-12"
                     /lab_host="Enterococcus faecalis"
                     /mol_type="other DNA"
                     /db_xref="taxon:478044"
     misc_feature    1..78
                     /label=multiple cloning region
                     /note="multiple cloning region"
     misc_feature    7..12
                     /label=SalI site
                     /note="SalI site"
     misc_feature    13..18
                     /label=SacI site
                     /note="SacI site"
     misc_feature    19..24
                     /label=BglII site
                     /note="BglII site"
     misc_feature    25..30
                     /label=BamHI site
                     /note="BamHI site"
     misc_feature    31..36
                     /label=KpnI site
                     /note="KpnI site"
     misc_feature    37..42
                     /label=SmaI site
                     /note="SmaI site"
     misc_feature    43..48
                     /label=SphI site
                     /note="SphI site"
     misc_feature    49..54
                     /label=XbaI site
                     /note="XbaI site"
     misc_feature    55..60
                     /label=EcoRI site
                     /note="EcoRI site"
     misc_feature    61..66
                     /label=XhoI site
                     /note="XhoI site"
     misc_feature    67..72
                     /label=EagI site
                     /note="EagI site"
     misc_feature    73..78
                     /label=HindIII site
                     /note="HindIII site"
     gene            complement(3245..3982)
                     /gene="erm"
                     /label=erm
     CDS             complement(3245..3982)
                     /codon_start=1
                     /gene="erm"
                     /product="Erm"
                     /label=erm
                     /note="erythromycin resistance"
                     /protein_id="ABW37926.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"
     regulatory      complement(3990..3994)
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(4038..4043)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(4065..4070)
                     /regulatory_class="minus_35_signal"