LOCUS       Exported                5274 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pAMPAT-MCS, complete sequence.
ACCESSION   AY436765
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Lipka V, Rademacher T, Panstruga R.
  TITLE     pAMPAT-MCS binary vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Lipka V, Rademacher T, Panstruga R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2003) Plant-Microbe Interaction, 
            Max-Planck-Institute for Plant Breeding Research, Carl-von-Linne-Weg
            10, Koeln D-50829, Germany
REFERENCE   3  (bases 1 to 5274)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Binary vector pAMPAT-MCS"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:254416"
     misc_feature    complement(join(1..10,5250..5274))
                     /note="T-DNA left border; LB"
     regulatory      complement(76..360)
                     /regulatory_class="terminator"
                     /note="pAnos"
     terminator      95..347
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(367..918)
                     /codon_start=1
                     /gene="pat"
                     /product="basta resistance protein"
                     /label=pat
                     /protein_id="AAR14725.1"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     gene            complement(367..918)
                     /gene="pat"
                     /label=pat
                     /note="Basta resistance"
     regulatory      complement(940..1250)
                     /regulatory_class="promoter"
                     /note="pnos"
     promoter        complement(961..1140)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     regulatory      1260..1958
                     /regulatory_class="promoter"
                     /note="p35S; double 35S promoter"
     promoter        1910..1956
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     1960..1976
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2010..2026)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2021..2238
                     /regulatory_class="terminator"
                     /note="pA35S"
     misc_feature    complement(2309..2334)
                     /note="T-DNA right border; RB"
     misc_feature    2309..2333
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      2445..3154
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      2449..3157
                     /label=RK2 origin
                     /note="RK2 origin"
     promoter        3228..3319
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3320..4180
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin/carbenicillin resistance"
                     /protein_id="AAR14724.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            3320..4180
                     /gene="bla"
                     /label=bla
     rep_origin      4249..4941
                     /label=ColE1 origin
                     /note="ColE1 origin"
     rep_origin      4351..4939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5251..1
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"