LOCUS       Exported                5694 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAMNPT-MCS, complete sequence.
ACCESSION   KJ433387
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Myrach T, Witte CP.
  TITLE     Improvement of urea use efficiency in Arabidopsis thaliana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Myrach T, Lipka V, Rademacher T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-2014) Department of Plant Biochemistry, Frei 
            Universitat Berlin, Dahlem Centre of Plant Sciences, 
            Koenigin-Luise-Str. 12-16, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 5694)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Cloning vector pAMNPT-MCS"
                     /mol_type="other DNA"
                     /db_xref="taxon:1488499"
     primer_bind     3..19
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(53..69)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      64..281
                     /regulatory_class="terminator"
                     /note="pA35S"
     misc_feature    312..457
                     /label=T-DNA right border
                     /note="T-DNA right border"
     misc_feature    352..376
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      488..1197
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      492..1200
                     /label=RK2
                     /note="RK2"
     promoter        1271..1362
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1363..2223
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance"
                     /protein_id="AHX24179.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            1363..2223
                     /gene="bla"
                     /label=bla
     rep_origin      2292..2984
                     /label=ColE1
                     /note="ColE1"
     rep_origin      2394..2982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3220..3363
                     /label=T-DNA left border
                     /note="T-DNA left border"
     misc_feature    3290..3314
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      complement(3372..3660)
                     /regulatory_class="terminator"
                     /note="pAOcs"
     CDS             complement(3834..4628)
                     /codon_start=1
                     /gene="npt"
                     /product="neomycin phosphotransferase"
                     /label=npt
                     /note="kanamycin resistance"
                     /protein_id="AHX24180.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            complement(3834..4628)
                     /gene="npt"
                     /label=npt
     regulatory      complement(4654..4989)
                     /regulatory_class="promoter"
                     /note="pnos"
     promoter        complement(4662..4845)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(4698..4877)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     regulatory      4997..5694
                     /regulatory_class="promoter"
                     /note="p35SS"
     promoter        5647..5693
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"