LOCUS       Exported                3654 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAmin81, complete sequence.
ACCESSION   HQ283398
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Hajimorad M, Gray PR, Keasling JD.
  TITLE     A framework and model system to investigate linear system behavior 
            in Escherichia coli
  JOURNAL   J Biol Eng 5 (1) (2011) In press
  PUBMED    21510907
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Hajimorad M, Gray PR, Keasling JD.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2010) Electrical Engineering and Computer 
            Sciences, University of California, Berkeley, 231 Cory Hall, 
            Berkeley, CA 94720, USA
REFERENCE   3  (bases 1 to 3654)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Cloning vector pAmin81"
                     /lab_host="Escherichia coli DH10B"
                     /mol_type="other DNA"
                     /db_xref="taxon:1036685"
     CDS             198..992
                     /codon_start=1
                     /product="neomycin phosphotransferase II"
                     /label=neomycin phosphotransferase II
                     /protein_id="AEG76964.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      1018..1123
                     /regulatory_class="terminator"
     terminator      1023..1117
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     1141..1157
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1179..1277
                     /regulatory_class="terminator"
     terminator      1185..1271
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      1319..3536
     CDS             complement(1837..2787)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2835..3057
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     regulatory      3549..3654
                     /regulatory_class="terminator"
     terminator      3554..3648
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"