LOCUS       Exported                5255 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Saccharomyces cerevisiae vector pAM656, complete sequence.
ACCESSION   KJ912895
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Agaphonov M, Alexandrov A.
  TITLE     Self-excising integrative yeast plasmid vectors containing an 
            intronated recombinase gene
  JOURNAL   FEMS Yeast Res. (2014) In press
  PUBMED    25124534
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Agaphonov M, Alexandrov A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2014) A.N. Bach Institute of Biochemistry, Russian
            Academy of Science, Moscow 119071, Russian Federation
REFERENCE   3  (bases 1 to 5255)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Saccharomyces cerevisiae vector pAM656"
                     /mol_type="other DNA"
                     /db_xref="taxon:1538092"
     primer_bind     complement(22..38)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     complement(51..84)
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(51..84)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        complement(109..550)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    433..550
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     CDS             join(681..683,735..1724)
                     /codon_start=1
                     /product="Cre"
                     /label=Cre
                     /protein_id="AIL02111.1"
                     /translation="MSIDATSDEVRKNLMDMFRDRQAFSEHTWKMLLSVCRSWAAWCKL
                     NNREWFPAEPEDVRGYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPRPSDSNAVSLVM
                     RRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLGIAYNTLLRIAE
                     IARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERWISVSGVADDPN
                     NYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRYLAWSGHSARVG
                     AARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDGD"
     intron          684..734
     rep_origin      complement(1797..2385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2556..3416)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AIL02112.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3417..3521)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3627..4029
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             4030..5124
                     /codon_start=1
                     /product="beta-isopropylmalate dehydrogenase"
                     /label=beta-isopropylmalate dehydrogenase
                     /protein_id="AIL02113.1"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     misc_recomb     complement(5195..5228)
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(5195..5228)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."