LOCUS       Exported                5014 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Hansenula polymorpha vector pAM631, complete sequence.
ACCESSION   KJ912897
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Agaphonov M, Alexandrov A.
  TITLE     Self-excising integrative yeast plasmid vectors containing an 
            intronated recombinase gene
  JOURNAL   FEMS Yeast Res. (2014) In press
  PUBMED    25124534
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Agaphonov M, Alexandrov A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2014) A.N. Bach Institute of Biochemistry, Russian
            Academy of Science, Moscow 119071, Russian Federation
REFERENCE   3  (bases 1 to 5014)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Hansenula polymorpha vector pAM631"
                     /mol_type="other DNA"
                     /db_xref="taxon:1538090"
     misc_recomb     complement(16..49)
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(16..49)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             join(548..550,602..1591)
                     /codon_start=1
                     /product="Cre"
                     /label=Cre
                     /protein_id="AIL02117.1"
                     /translation="MSIDATSDEVRKNLMDMFRDRQAFSEHTWKMLLSVCRSWAAWCKL
                     NNREWFPAEPEDVRGYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPRPSDSNAVSLVM
                     RRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLGIAYNTLLRIAE
                     IARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERWISVSGVADDPN
                     NYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRYLAWSGHSARVG
                     AARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDGD"
     intron          551..601
     rep_origin      complement(1664..2252)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2423..3283)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AIL02118.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3284..3388)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3720..4814
                     /codon_start=1
                     /product="beta-isopropylmalate dehydrogenase"
                     /label=beta-isopropylmalate dehydrogenase
                     /protein_id="AIL02119.1"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     misc_recomb     complement(4889..4922)
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(4889..4922)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."