LOCUS       Exported                3410 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAM434, complete sequence.
ACCESSION   AF435436
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Flannagan SE, Clewell DB.
  TITLE     Identification and characterization of genes encoding sex pheromone 
            cAM373 activity in Enterococcus faecalis and Staphylococcus aureus
  JOURNAL   Mol. Microbiol. 44 (3), 803-817 (2002)
  PUBMED    11994160
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Flannagan SE, Clewell DB.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2001) School of Dentistry, The University of 
            Michigan, 1011 N. University Ave., Ann Arbor, MI 48109-1078, USA
REFERENCE   3  (bases 1 to 3410)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Cloning vector pAM434"
                     /lab_host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:192850"
     misc_feature    1..58
                     /label=cloning region
                     /note="cloning region"
     promoter        complement(40..58)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(79..95)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    103..119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(127..157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    172..193
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(481..1069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1474..1490
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1500..1626
                     /label=cloning region
                     /note="cloning region"
     promoter        1500..1518
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1579..1595
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            2454..3279
                     /gene="erm(B)"
                     /label=erm(B)
     regulatory      2454..2459
                     /regulatory_class="minus_35_signal"
                     /gene="erm(B)"
     regulatory      2481..2486
                     /regulatory_class="minus_10_signal"
                     /gene="erm(B)"
     regulatory      2530..2534
                     /regulatory_class="ribosome_binding_site"
                     /gene="erm(B)"
     CDS             2542..3279
                     /codon_start=1
                     /gene="erm(B)"
                     /product="Erm(B)"
                     /label=erm(B)
                     /note="erythromycin resistance protein; Erm(B)"
                     /protein_id="AAM26278.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"