LOCUS       Exported                8384 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pAM2770, complete sequence.
ACCESSION   AY265466
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Lee MH, Scherer M, Rigali S, Golden JW.
  TITLE     PlmA, a member of the GntR family, has plasmid maintenance functions
            in Anabaena sp. strain PCC 7120
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8384)
  AUTHORS   Lee MH.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2003) Biology, Texas A&M University, 3258 TAMU, 
            College Station, TX 77840, USA
REFERENCE   3  (bases 1 to 8384)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Shuttle vector pAM2770"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:227719"
     CDS             739..1533
                     /codon_start=1
                     /gene="npt"
                     /product="neomycin phosphotransferase"
                     /label=npt
                     /note="Nm/Km resistance marker"
                     /protein_id="AAO92599.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            739..1533
                     /gene="npt"
                     /label=npt
     misc_feature    2086..2228
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2414..3002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3103..3120)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(3144..3467)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta galactosidase alpha peptide"
                     /label=lacZ
                     /note="NdeI site has been removed by silent, site directed 
                     mutagenesis; N-terminal fragment; alpha (delta NdeI)"
                     /protein_id="AAO92600.1"
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     gene            complement(3144..3467)
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     3377..3393
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3397..3453
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     regulatory      complement(3467..3841)
                     /regulatory_class="promoter"
                     /note="copper-inducible petE promoter"
     CDS             5291..6412
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="required for replication"
                     /protein_id="AAO92601.1"
                     /translation="MISPESLNTNVSLTLLTRELQTEIILGTYTVRVHTRIGREPCARL
                     WYLCRALDKDGSGHLTLPLPVVQTFLDCSDKSVYRWLQDGKKIGAFRRYKIKAGMITVY
                     LGGMFQVCYNLNLKRWGDVAVVPLVQVLSDLRSLTTGIVTQSFQQKSRYAANRQLKPEY
                     RKLFGAPHPNELVKDTRQSSLKSPEGEVPCVLHISSSRIFVSKSFIHYGTSQKAVSCEL
                     GIHKRTVRRHQKQLGMNRRQLCQAKIEYNQLRHARNNDASEFWAFTGTKTDIGYQVMGD
                     AVVFSDGIAPGAKKRQPNTYQIDATEFDGRLFKVGDKVFMNRCNIYREQFTLTTMSAAR
                     RKYHFKLSQCHFSENRAGRVGNRFVIGCHSGEI"
     gene            5291..6412
                     /gene="repA"
                     /label=repA
     CDS             complement(7033..7584)
                     /codon_start=1
                     /gene="int/res"
                     /product="integrase/resolvase"
                     /label=int/res
                     /protein_id="AAO92602.1"
                     /translation="MKVNGNGRAKILTSDELRRLFSDGFTTPRDRVLFGICLFTGCRVS
                     EALALQTTDIKGETLTFRKSTTKGKLKTRVVDIQPGLAALMADYHPKPGTLFPGMRGVS
                     DRLTRYAADKILRDAAKRIGLEGISTHSFRRTALNQMSSAGIPLRHIQEISGHNDLGTL
                     QRYLEVTPEQRRKAVSVIGF"
     gene            complement(7033..7584)
                     /gene="int/res"
                     /label=int/res