LOCUS       Exported               10577 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAM1956, complete sequence.
ACCESSION   KJ755972
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10577)
  AUTHORS   Videau P, Callahan SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2014) Microbiology, University of Hawaii, 2538 
            McCarthy Mall, Snyder Hall 201, Honolulu, HI 96822, USA
REFERENCE   2  (bases 1 to 10577)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Gene Construction Kit v. 3.5
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10577
                     /organism="Cloning vector pAM1956"
                     /mol_type="other DNA"
                     /db_xref="taxon:1522258"
     CDS             6744..7460
                     /codon_start=1
                     /product="GFP"
                     /label=GFP
                     /protein_id="AIG59961.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     rep_origin      7584..8172
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    8358..8498
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(9045..9839)
                     /codon_start=1
                     /product="NptII"
                     /label=NptII
                     /note="confers neomycin/kanamycin resistance"
                     /protein_id="AIG59962.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"