LOCUS       Exported               12248 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pALH122, complete sequence.
ACCESSION   AF405697
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12248)
  AUTHORS   Honeyman AL, Cote CK, Curtiss R III.
  TITLE     Construction of transcriptional and translational lacZ gene reporter
            plasmids for use in Streptococcus mutans
  JOURNAL   J. Microbiol. Methods 49 (2), 163-171 (2002)
  PUBMED    11830302
REFERENCE   2  (bases 1 to 12248)
  AUTHORS   Honeyman AL, Cote CK, Curtiss R III.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2001) Department of Medical Microbiology and 
            Immunology, University of South Florida, 12901 Bruce B. Downs Blvd.,
            Tampa, FL 33612, USA
REFERENCE   3  (bases 1 to 12248)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12248
                     /organism="Reporter vector pALH122"
                     /lab_host="Streptococcus mutans"
                     /mol_type="other DNA"
                     /db_xref="taxon:187307"
     CDS             complement(652..3726)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /note="derived from Escherichia coli"
                     /protein_id="AAN02497.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(652..3726)
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     3690..3706
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(3735..3741)
                     /regulatory_class="ribosome_binding_site"
                     /note="lacZ modified"
     misc_feature    complement(3741..3743)
                     /label=stop codon
                     /note="stop codon"
     misc_feature    complement(3745..3747)
                     /label=stop codon
                     /note="stop codon"
     misc_feature    complement(3749..3751)
                     /label=stop codon
                     /note="stop codon"
     CDS             complement(5465..6124)
                     /codon_start=1
                     /gene="cam"
                     /product="chloramphenicol resistance protein"
                     /label=cam
                     /note="derived from pACYC184"
                     /protein_id="AAN02498.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(5465..6124)
                     /gene="cam"
                     /label=cam
     promoter        complement(6125..6227)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      complement(6753..7298)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             9260..9973
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label=rep
                     /note="derived from pVA380-1; required for Gram positive 
                     hosts"
                     /protein_id="AAN02499.1"
                     /translation="MKYAYQAELVVNEAMKRYPKGRFLFLTLTIKNISGEKLNKSISEI
                     GRAFNRLMKYKKVDKNVIGYLRATEVTYSTEHENYHPHLHVLLFVKSSYFTGNNTNYIS
                     QEEWTKLWAKAMKLDYTPVVDIRTVKAHKRKNLKSAIIETAKYPVKPFDVDTEDVTLFS
                     EMVKERITEDLTNGLHRKRQIGFGKLFKKIKAELALDDVEEGNLVQTGAEESAESTGRE
                     IVAFWNWDRKNYFVR"
     gene            9260..9973
                     /gene="rep"
                     /label=rep
     CDS             complement(10598..11335)
                     /codon_start=1
                     /gene="ery"
                     /product="erythromycin resistance protein"
                     /label=ery
                     /note="provides erythromycin resistance in Gram positive 
                     hosts"
                     /protein_id="AAN02500.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(10598..11335)
                     /gene="ery"
                     /label=ery