LOCUS       Exported                6815 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Integrating expression vector pALE3, complete sequence.
ACCESSION   DQ068699
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Fortune SM, Jaeger A, Sarracino DA, Chase MR, Sassetti CM, Sherman 
            DR, Bloom BR, Rubin EJ.
  TITLE     Mutually-dependent secretion of proteins required for mycobacterial 
            virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Fortune SM, Jaeger A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2005) Immunology and Infectious Diseases, Harvard 
            School of Public Health, 200 Longwood Ave., Armenise 439, Boston, MA
            02139, USA
REFERENCE   3  (bases 1 to 6815)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Integrating expression vector pALE3"
                     /mol_type="other DNA"
                     /label=derived from Mycobacterium tuberculosis
                     /note="derived from Mycobacterium tuberculosis"
                     /db_xref="taxon:332514"
     terminator      52..138
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      230..257
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     5UTR           295..796
     CDS             797..1975
                     /codon_start=1
                     /gene="Rv3616c"
                     /product="Rv3616c"
                     /label=Rv3616c
                     /note="kanamycin resistance"
                     /protein_id="AAY84559.1"
                     /translation="MSRAFIIDPTISAIDGLYDLLGIGIPNQGGILYSSLEYFEKALEE
                     LAAAFPGDGWLGSAADKYAGKNRNHVNFFQELADLDRQLISLIHDQANAVQTTRDILEG
                     AKKGLEFVRPVAVDLTYIPVVGHALSAAFQAPFCAGAMAVVGGALAYLVVKTLINATQL
                     LKLLAKLAELVAAAIADIISDVADIIKGTLGEVWEFITNALNGLKELWDKLTGWVTGLF
                     SRGWSNLESFFAGVPGLTGATSGLSQVTGLFGAAGLSASSGLAHADSLASSASLPALAG
                     IGGGSGFGGLPSLAQVHAASTRQALRPRADGPVGAAAEQVGGQSQLVSAQGSQGMGGPV
                     GMGGMHPSSGASKGTTTKKYSEGAAAGTEDAERAPVEADAGGGQKVLVRNVV"
     gene            797..1975
                     /gene="Rv3616c"
                     /label=Rv3616c
     terminator      2019..2066
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    2115..2901
                     /label=OriE
                     /note="OriE"
     rep_origin      complement(2270..2858)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3190..4005)
                     /codon_start=1
                     /gene="aph(3)-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"