LOCUS       Exported                5786 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAJ63a, complete sequence.
ACCESSION   JX101335
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Peters B, Junker A, Brauer K, Muhlthaler B, Kostner D, Mientus M, 
            Liebl W, Ehrenreich A.
  TITLE     Deletion of pyruvate decarboxylase by a new method for efficient 
            markerless gene deletions in Gluconobacter oxydans
  JOURNAL   Appl. Microbiol. Biotechnol. 97 (6), 2521-2530 (2013)
  PUBMED    22940799
REFERENCE   2  (bases 1 to 5786)
  AUTHORS   Peters B, Junker A, Brauer K, Kostner D, Mientus M, Liebl W, 
            Ehrenreich A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2012) Department of Microbiology, Technische 
            Universitaet Muenchen, Emil-Ramann-Str. 4, Freising, Bayern 85354, 
            Germany
REFERENCE   3  (bases 1 to 5786)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Cloning vector pAJ63a"
                     /mol_type="other DNA"
                     /db_xref="taxon:1219860"
     CDS             401..1195
                     /codon_start=1
                     /gene="aph(3`)-IIa"
                     /product="kanamycin resistance protein"
                     /label=aph(3`)-IIa
                     /protein_id="AFP65782.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            401..1195
                     /gene="aph(3`)-IIa"
                     /label=aph(3`)-IIa
     CDS             1888..2526
                     /codon_start=1
                     /gene="upp"
                     /product="UPRTase"
                     /EC_number="2.4.2.9"
                     /label=upp
                     /note="uracil phosphoribosyltransferase; derived from 
                     Gluconobacter oxydans 621H"
                     /protein_id="AFP65781.1"
                     /translation="MSQALPPIVLDHPLVRHKLTRLRDHNTSTAGFRRLTRELSLLLAY
                     EATRNLSLAPRHIEAPSGPMEGEELDGKKLCFVSILRAGNGLLDGMLDLVPSARVGHIG
                     LRRDHETLEVSEYYFNMPSDVPGRTCIVLDPMLATGHSAAAALTRVKAAGATNPIFACL
                     LAVPEGIAHMAELHPDVQIVTCSIDSHLDEHGYIVPGLGDAGDRLFGTR"
     gene            1888..2526
                     /gene="upp"
                     /label=upp
     oriT            3529..3638
                     /note="incP origin of transfer"
     promoter        complement(3873..3963)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        complement(4157..4247)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      4607..5195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5195
                     /label=oriV of pk18mobGII
                     /note="oriV of pk18mobGII"
     protein_bind    5483..5504
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5519..5549
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5557..5573
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5581..5597
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5606..5662
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5666..5682)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"