LOCUS       Exported                5064 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAJ50-2, complete sequence.
ACCESSION   KY852489
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Shao H, Li X, Lok JB.
  TITLE     Heritable genetic transformation of Strongyloides stercoralis by 
            microinjection of plasmid DNA constructs into the male germline
  JOURNAL   Int. J. Parasitol. (2017) In press
  PUBMED    28577882
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Shao H, Li X, Lok JB.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAR-2017) Pathobiology, University of Pennsylvania, 
            3800 Spruce Street, Rosenthal 212, Philadelphia, PA 19104, USA
REFERENCE   3  (bases 1 to 5064)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Cloning vector pAJ50-2"
                     /mol_type="other DNA"
                     /db_xref="taxon:2008618"
     protein_bind    96..117
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        132..162
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    170..186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     194..210
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      346..1508
                     /regulatory_class="promoter"
     CDS             1513..2211
                     /codon_start=1
                     /gene="mRFPmars"
                     /product="red fluorescent protein"
                     /label=mRFPmars
                     /note="RFP"
                     /protein_id="ARW80046.1"
                     /translation="MASSEDVIKEFMRFKVKMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERIMNFEDGGV
                     VTVTQDSTLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYKAKKQVQLPGAYKTDIKLDIISHNEDYTIVEQYERAEGRHS
                     TGAGSPRNLF"
     gene            1513..2211
                     /gene="mRFPmars"
                     /label=mRFPmars
     3UTR           2212..2763
                     /gene="Ss era-1"
     gene            2212..2763
                     /gene="Ss era-1"
                     /label=Ss era-1
     promoter        3148..3252
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3253..4113
                     /codon_start=1
                     /gene="ampR"
                     /product="ampicillin resistance protein"
                     /label=ampR
                     /protein_id="ARW80047.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            3253..4113
                     /gene="ampR"
                     /label=ampR
     rep_origin      4284..4872
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"