LOCUS       Exported                9021 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Tn917 delivery vector pAJ005, complete sequence.
ACCESSION   AF061336
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Framson PE, Nittayajarn A, Merry J, Youngman P, Rubens CE.
  TITLE     New genetic techniques for group B streptococci: high-efficiency 
            transformation, maintenance of temperature-sensitive pWV01 plasmids,
            and mutagenesis with Tn917
  JOURNAL   Appl. Environ. Microbiol. 63 (9), 3539-3547 (1997)
  PUBMED    9293004
REFERENCE   2  (bases 1 to 9021)
  AUTHORS   Jones AL, Rubens CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-1998) Pediatrics, Childrens Hospital and Regional
            Medical Center, 4800 Sand Point Way N.E., Seattle, WA 98105, USA
REFERENCE   3  (bases 1 to 9021)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9021
                     /organism="Tn917 delivery vector pAJ005"
                     /lab_host="Streptococcus agalactiae A909"
                     /mol_type="other DNA"
                     /note="temperature sensitive vector derived from pTV10K 
                     plasmid"
                     /db_xref="taxon:76156"
     mobile_element  264..5535
                     /mobile_element_type="transposon:Tn917"
     RBS             3821..3829
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3 end of the 16S rRNA (Chen et al., 1994)"
     gene            4167..4929
                     /gene="erm"
                     /label=erm
                     /note="inducible"
     CDS             complement(4167..4928)
                     /codon_start=1
                     /product="erythromycin resistance determinant"
                     /label=erythromycin resistance determinant
                     /protein_id="AAC16049.1"
                     /translation="MYHFRSDYMNKNIKYSQNFLTNEKVLNQIIKQLNLKETDTVYEIG
                     TGKGHLTTKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYK
                     IVGNIPYHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLL
                     KLPAECFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQA
                     MKHAKVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            6078..6873
                     /gene="aphA3"
                     /label=aphA3
     CDS             6079..6873
                     /codon_start=1
                     /gene="aphA3"
                     /product="aminoglycoside phosphotransferase A3"
                     /label=aphA3
                     /protein_id="AAC16050.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             complement(7277..7975)
                     /codon_start=1
                     /product="temperature-sensitive RepA"
                     /label=temperature-sensitive RepA
                     /protein_id="AAC16051.1"
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNNSNIIQNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
     gene            7277..7975
                     /gene="repAts"
                     /label=repAts
     CDS             complement(8042..8203)
                     /codon_start=1
                     /product="RepC"
                     /label=RepC
                     /protein_id="AAC16052.1"
                     /translation="MVISESKKRVMISLTKEQDKKLTDMAKQKGFSKSAVAALAIEEYA
                     RKESEQKK"
     gene            8042..8203
                     /gene="repC"
                     /label=repC
     CDS             8587..8598
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"