LOCUS       Exported               10468 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  PtGAUT12 RNAi binary vector pAGW641, complete sequence.
ACCESSION   KP259612
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Biswal AK, Hao Z, Pattathil S, Yang X, Winkeler K, Collins C, 
            Mohanty SS, Richardson EA, Gelineo-Albersheim I, Hunt K, Ryno D, 
            Sykes RW, Turner GB, Ziebell A, Gjersing E, Lukowitz W, Davis MF, 
            Decker SR, Hahn MG, Mohnen D.
  TITLE     Downregulation of GAUT12 in Populus deltoides by RNA silencing 
            results in reduced recalcitrance, increased growth and reduced xylan
            and pectin in a woody biofuel feedstock
  JOURNAL   Biotechnol Biofuels 8, 41 (2015)
  PUBMED    25802552
REFERENCE   2  (bases 1 to 10468)
  AUTHORS   Rottmann WH, Winkeler KA, Biswal AK, Yang X.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2014) ArborGen Inc., 2011 Broadbank Ct., 
            Ridgeville, SC 29472, USA
REFERENCE   3  (bases 1 to 10468)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Apr 14, 2015 this sequence version replaced KP259612.1.
FEATURES             Location/Qualifiers
     source          1..10468
                     /organism="PtGAUT12 RNAi binary vector pAGW641"
                     /mol_type="other DNA"
                     /db_xref="taxon:1634916"
     CDS             802..1596
                     /codon_start=1
                     /product="NptIII"
                     /label=NptIII
                     /note="kanamycin resistance"
                     /protein_id="AKA27928.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             1895..3043
                     /codon_start=1
                     /product="trfA"
                     /label=trfA
                     /note="functions in plasmid replication"
                     /protein_id="AKA27929.1"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    3495..3519
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    3496..3519
                     /label=left T-DNA border
                     /note="left T-DNA border"
     primer_bind     3857..3873
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            complement(3875..6262)
                     /gene="nos"
                     /label=nos
     regulatory      complement(3875..4095)
                     /regulatory_class="terminator"
                     /gene="nos"
                     /note="nopaline synthase (nos) terminator"
     terminator      3886..4138
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4141..4935)
                     /codon_start=1
                     /gene="nos"
                     /product="neomycin phosphotransferase"
                     /label=nos
                     /note="kanamycin resistance"
                     /protein_id="AKA27930.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      complement(4936..6262)
                     /regulatory_class="promoter"
                     /gene="nos"
                     /note="Arabidopsis thaliana UBIQUITIN10 (At4g05320) 
                     promoter plus 5-UTR"
     terminator      6300..6552
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    complement(6303..10289)
                     /note="inverted repeat of a fragment of PtGAUT12 
                     (POPTR_0001s44250, Potri.001G416800)"
     regulatory      complement(6303..6502)
                     /regulatory_class="terminator"
                     /note="nopaline synthase (nos) terminator"
     misc_feature    6610..6634
                     /label=attB1
                     /note="attB1"
     protein_bind    6610..6634
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6661..6828
                     /label=fragment of PtGAUT12
                     /note="fragment of PtGAUT12"
     misc_feature    complement(6851..6875)
                     /label=attB2
                     /note="attB2"
     protein_bind    complement(6851..6875)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(6904..8243)
                     /note="spacer fragment from Petunia hybrida chalcone 
                     synthase (chsA; INSD accession X14591)"
     misc_feature    8262..8286
                     /label=attB2
                     /note="attB2"
     protein_bind    8262..8286
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(8309..8476)
                     /label=fragment of PtGAUT12
                     /note="fragment of PtGAUT12"
     misc_feature    complement(8503..8527)
                     /label=attB1
                     /note="attB1"
     protein_bind    complement(8503..8527)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      complement(8550..10289)
                     /regulatory_class="promoter"
                     /note="Arabidopsis thaliana UBIQUITIN3 (At5g03240) promoter
                     plus 5-UTR"
     misc_feature    10335..10359
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    10336..10359
                     /label=right T-DNA border
                     /note="right T-DNA border"