LOCUS       Exported               10326 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pAGW633, complete sequence.
ACCESSION   KX683862
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10326)
  AUTHORS   Biswal AK, Atmodjo MA, Li M, Yoo CG, Pu Y, Lee Y-C., Zhang J-Y., 
            Ramanna H, Bray AL, King ZR, LaFayette PR, Mohanty SS, Ryno D, Yee 
            K, Thompson OA, Rodriguez M Jr., Winkeler KA, Collins C, Yang X, Tan
            L, Sykes RW, Gjersing EL, Ziebell A, Turner GB, Decker SR, Parrott 
            WA, Udvardi MK, Mielenz J, Davis MF, Nelson RS, Ragauskas AJ, Mohnen
            D.
  TITLE     Targeted modification of pectin biosynthesis to improve growth and 
            deconstruction of woody and grass bioenergy feedstocks
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10326)
  AUTHORS   Rottmann WH, Winkeler KA, Biswal AK, Yang X.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2016) Biochemistry and Molecular Biology, Complex 
            Carbohydrate Research Center, The University of Georgia, 315 
            Riverbend Rd, Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 10326)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10326
                     /organism="Binary vector pAGW633"
                     /mol_type="other DNA"
                     /db_xref="taxon:2016464"
     CDS             801..1595
                     /codon_start=1
                     /product="NptIII"
                     /label=NptIII
                     /note="kanamycin resistance protein"
                     /protein_id="ASJ77177.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             1894..3042
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    3399..3423
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    3400..3423
                     /label=left T-DNA border
                     /note="left T-DNA border"
     primer_bind     3761..3777
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            complement(3779..6166)
                     /gene="nos"
                     /label=nos
     regulatory      complement(3779..3999)
                     /regulatory_class="terminator"
                     /gene="nos"
                     /note="nopaline synthase (nos) terminator"
     terminator      3790..4042
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4045..4839)
                     /codon_start=1
                     /gene="nos"
                     /product="neomycin phosphotransferase"
                     /label=nos
                     /note="kanamycin resistance protein"
                     /protein_id="ASJ77178.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    complement(4840..6166)
                     /gene="nos"
                     /note="Arabidopsis thaliana ubiquitni 10 (At4g05320) 
                     promoter and 5 UTR"
     terminator      6204..6456
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     repeat_region   6207..10147
                     /label=inverted repeat of a fragment of PtGAUT4
                     /note="inverted repeat of a fragment of PtGAUT4"
     regulatory      complement(6207..6406)
                     /regulatory_class="terminator"
                     /note="nopaline synthase (nos) terminator"
     protein_bind    6514..6538
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     complement(6515..6535)
                     /label=attB1
                     /note="attB1"
     misc_feature    complement(6565..6714)
                     /label=fragment of PtGAUT4
                     /note="fragment of PtGAUT4"
     protein_bind    complement(6732..6756)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     complement(6735..6755)
                     /label=attB2
                     /note="attB2"
     misc_feature    complement(6785..8124)
                     /note="spacer fragment from intron of Petunia hybrida 
                     chalcone synthase (chsA) INSD accession X14591"
     protein_bind    8143..8167
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     complement(8144..8164)
                     /label=attB2
                     /note="attB2"
     misc_feature    complement(8185..8334)
                     /label=fragment of PtGAUT4
                     /note="fragment of PtGAUT4"
     protein_bind    complement(8361..8385)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     complement(8364..8384)
                     /label=attB1
                     /note="attB1"
     misc_feature    complement(8408..10147)
                     /note="Arabidopsis thaliana ubiquitin 3 (At5g03240) 
                     promoter and 5 UTR"
     misc_feature    10193..10217
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    10194..10217
                     /label=right T-DNA border
                     /note="right T-DNA border"