LOCUS       Exported               12660 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Binary RNAi Gateway destination vector pAGSM552, complete sequence.
ACCESSION   KP259613
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12660)
  AUTHORS   Rottmann WH, Miller SA.
  TITLE     Plasmids used by BioEnergy Science Center (BESC) for Populus 
            transformation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12660)
  AUTHORS   Rottmann WH, Miller SA.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2014) ArborGen Inc., 2011 Broadbank Ct., 
            Ridgeville, SC 29472, USA
REFERENCE   3  (bases 1 to 12660)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Apr 14, 2015 this sequence version replaced KP259613.1.
FEATURES             Location/Qualifiers
     source          1..12660
                     /organism="Binary RNAi Gateway destination vector
                     pAGSM552"
                     /mol_type="other DNA"
                     /db_xref="taxon:1634915"
     CDS             802..1596
                     /codon_start=1
                     /product="NptIII"
                     /label=NptIII
                     /note="kanamycin resistance"
                     /protein_id="AKA27932.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             1895..3043
                     /codon_start=1
                     /product="TrfA"
                     /label=TrfA
                     /note="functions in plasmid replication"
                     /protein_id="AKA27933.1"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    3495..3519
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    3496..3519
                     /label=left T-DNA border
                     /note="left T-DNA border"
     primer_bind     3857..3873
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            complement(3875..6262)
                     /gene="nos"
                     /label=nos
     regulatory      complement(3875..4095)
                     /regulatory_class="terminator"
                     /gene="nos"
                     /note="nopaline synthase (nos) terminator"
     terminator      3886..4138
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4141..4935)
                     /codon_start=1
                     /gene="nos"
                     /product="neomycin phosphotransferase"
                     /label=nos
                     /note="UBQ10; kanamycin resistance"
                     /protein_id="AKA27931.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      complement(4936..6262)
                     /regulatory_class="promoter"
                     /gene="nos"
                     /note="Arabidopsis thaliana UBIQUITIN10 (At4g05320) 
                     promoter plus 5-UTR"
     intron          complement(4936..5239)
                     /gene="nos"
     terminator      6300..6552
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      complement(6303..6502)
                     /regulatory_class="terminator"
                     /note="nopaline synthase (nos) terminator"
     misc_feature    6610..6734
                     /label=attR1
                     /note="attR1"
     protein_bind    6610..6734
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6759..6789
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             complement(6852..7157)
                     /codon_start=1
                     /product="CcdB"
                     /label=CcdB
                     /note="cytotoxic protein"
                     /protein_id="AKA27934.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     misc_feature    complement(7505..7629)
                     /label=attR2
                     /note="attR2"
     protein_bind    complement(7505..7629)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    complement(7657..8996)
                     /note="spacer fragment from Petunia hybrida chalcone 
                     synthase (chsA; INSD accession X14591)"
     misc_feature    9015..9139
                     /label=attR2
                     /note="attR2"
     protein_bind    9015..9139
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(9180..9485)
                     /codon_start=1
                     /product="CcdB"
                     /label=CcdB
                     /note="cytotoxic protein"
                     /protein_id="AKA27935.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(9827..10486)
                     /codon_start=1
                     /product="CmR"
                     /label=CmR
                     /note="chloramphenicol resistance"
                     /protein_id="AKA27936.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(10540..10570)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     misc_feature    complement(10595..10719)
                     /label=attR1
                     /note="attR1"
     protein_bind    complement(10595..10719)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      complement(10753..12481)
                     /regulatory_class="promoter"
                     /note="Arabidopsis thaliana UBIQUITIN3 (At5g03240) promoter
                     plus 5-UTR"
     intron          complement(10753..11127)
     misc_feature    12527..12551
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    12528..12551
                     /label=right T-DNA border
                     /note="right T-DNA border"