LOCUS       Exported                3018 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAGA3, complete sequence.
ACCESSION   AY452085
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Hu HZ, Gu Q, Wang C, Colton CK, Tang J, Kinoshita-Kawada M, Lee LY, 
            Wood JD, Zhu MX.
  TITLE     2-aminoethoxydiphenyl borate is a common activator of TRPV1, TRPV2, 
            and TRPV3
  JOURNAL   J. Biol. Chem. 279 (34), 35741-35748 (2004)
  PUBMED    15194687
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Zhu MX.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2003) Neuroscience, The Ohio State University, 168
            Rightmire Hall, 1060 Carmack Road, Columbus, OH 43210, USA
REFERENCE   3  (bases 1 to 3018)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Cloning vector pAGA3"
                     /mol_type="other DNA"
                     /label=prokaryotic expression vector
                     /note="prokaryotic expression vector"
                     /db_xref="taxon:262787"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    33..66
                     /note="5 untranslated region of alfalfa mosaic virus RNA 
                     4"
     misc_feature    67..114
                     /label=polylinker
                     /note="polylinker"
     misc_feature    115..206
                     /label=polyA tail
                     /note="polyA tail"
     promoter        complement(239..257)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        1043..1147
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1148..2008
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="AmpR"
                     /protein_id="AAS17530.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2179..2767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"