LOCUS       Exported                4035 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  GlnQ expression vector pAG200, complete sequence.
ACCESSION   AF372621
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Tamura GS, Nittayajarn A, Schoentag DL.
  TITLE     A glutamine transport gene, glnQ, is required for fibronectin 
            adherence and virulence of group B streptococci
  JOURNAL   Infect. Immun. 70 (6), 2877-2885 (2002)
  PUBMED    12010975
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Tamura GS, Schoentag DL, Nittayajarn A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2001) Department of Pediatrics, Division of 
            Infectious Diseases, Childrens Hospital and Regional Medical Center
            and the University of Washington, 4800 Sand Point Way NE, Seattle, 
            WA 98105-0371, USA
REFERENCE   3  (bases 1 to 4035)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="glnQ expression vector pAG200"
                     /plasmid="pAG200"
                     /lab_host="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:159744"
     CDS             complement(136..876)
                     /codon_start=1
                     /gene="glnQ"
                     /product="GlnQ"
                     /label=glnQ
                     /note="ATP-binding component"
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="AAK57388.1"
                     /translation="MAELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGKS
                     TFLRTLNLLESITSGKVVVDGFELSNPKTDIDKARENIGMVFQHFNLFPHMSVLENITF
                     APIELGKESKEAAEKHGMELLEKVGLADKANAKPDSLSGGQKQRVAIARSLAMNPDILL
                     FDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGRFLEDG
                     TPEQIFDTPQHPRLQDFLNKVLNV"
     gene            complement(136..876)
                     /gene="glnQ"
                     /label=glnQ
     CDS             377..394
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     CDS             complement(1101..1751)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyl transferase"
                     /label=cat
                     /protein_id="AAK57387.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            complement(1101..1751)
                     /gene="cat"
                     /label=cat