LOCUS       Exported                5126 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  GlnQ allelic exchange vector pAG101, complete sequence.
ACCESSION   AF372620
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Tamura GS, Nittayajarn A, Schoentag DL.
  TITLE     A glutamine transport gene, glnQ, is required for fibronectin 
            adherence and virulence of group B streptococci
  JOURNAL   Infect. Immun. 70 (6), 2877-2885 (2002)
  PUBMED    12010975
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Tamura GS, Schoentag DL, Nittayajarn A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2001) Department of Pediatrics, Division of 
            Infectious Diseases, Childrens Hospital and Regional Medical Center
            and the University of Washington, 4800 Sand Point Way NE, Seattle, 
            WA 98105-0371, USA
REFERENCE   3  (bases 1 to 5126)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="glnQ allelic exchange vector pAG101"
                     /plasmid="pAG101"
                     /lab_host="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /label=temperature-sensitive plasmid
                     /note="temperature-sensitive plasmid"
                     /db_xref="taxon:159742"
     CDS             102..839
                     /codon_start=1
                     /gene="erm"
                     /product="ribosomal RNA methylase"
                     /label=erm
                     /note="confers erythromycin-resistance"
                     /protein_id="AAK57385.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"
     gene            102..839
                     /gene="erm"
                     /label=erm
     misc_feature    859..1116
                     /label=glnQ gene interrupted by erm gene
                     /note="glnQ gene interrupted by erm gene"
     CDS             complement(879..896)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(1135..1151)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1601..1612)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             3845..4495
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyl transferase"
                     /label=cat
                     /note="confers chloramphenicol resistance"
                     /protein_id="AAK57386.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            3845..4495
                     /gene="cat"
                     /label=cat
     primer_bind     4665..4681
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4688..4706
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4732..4748
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4747..5126
                     /label=glnQ gene interrupted by erm gene
                     /note="glnQ gene interrupted by erm gene"