LOCUS       Exported               10000 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pADNpcDNA3.1 KanR, complete sequence.
ACCESSION   KX176867
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Ambati S, Yu P, McKinney EC, Kandasamy MK, Hartzell D, Baile CA, 
            Meagher RB.
  TITLE     Adipocyte nuclei captured from VAT and SAT
  JOURNAL   BMC Obes 3, 35 (2016)
  PUBMED    27462403
REFERENCE   2  (bases 1 to 10000)
  AUTHORS   Ambati S, Yu P, McKinney EC, Kandasamy MK, Hartzell D, Baile CA, 
            Meagher RB.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2016) Genetics, University of Georgia, 120 East 
            Green Street, Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 10000)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10000
                     /organism="Cloning vector pADNpcDNA3.1 KanR"
                     /mol_type="other DNA"
                     /db_xref="taxon:1884655"
     regulatory      37..5486
                     /regulatory_class="promoter"
                     /note="ADNp*"
     misc_feature    2820..2825
                     /label=AgeI site
                     /note="AgeI site"
     misc_feature    5487..5548
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      5574..5849
                     /regulatory_class="other"
                     /note="BGH polyadenylation signal sequence"
     polyA_signal    5580..5804
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5850..6278
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     oriT            5850..6263
                     /label=f1
                     /note="f1"
     promoter        6292..6621
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      6319..6652
                     /regulatory_class="other"
                     /note="early promoter and origin"
     rep_origin      6472..6607
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6688..7482
                     /codon_start=1
                     /gene="neoR"
                     /product="NeoR"
                     /label=neoR
                     /protein_id="ANZ72460.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            6688..7482
                     /gene="neoR"
                     /label=neoR
     polyA_signal    7656..7777
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      7656..7769
                     /regulatory_class="other"
                     /note="SV40 polyadenylation signal sequence"
     primer_bind     complement(7826..7842)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7850..7866
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7874..7904)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7919..7940
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      8191..8857
                     /label=pUC Ori
                     /note="pUC Ori"
     rep_origin      complement(8248..8833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9004..9864)
                     /codon_start=1
                     /gene="ampR"
                     /product="AmpR"
                     /label=ampR
                     /protein_id="ANZ72459.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(9004..9864)
                     /gene="ampR"
                     /label=ampR
     promoter        complement(9865..9969)
                     /gene="bla"
                     /label=AmpR promoter