LOCUS       Exported                7117 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pADH33, complete sequence.
ACCESSION   KC202163
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Hernday A.
  TITLE     Structure of the Transcriptional Network Controlling White-Opaque 
            Switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7117)
  AUTHORS   Hernday A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-NOV-2012) Microbiology and Immunology, UCSF, 600 16th 
            St, San Francisco, CA 94107, USA
REFERENCE   3  (bases 1 to 7117)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="Cloning vector pADH33"
                     /mol_type="other DNA"
                     /db_xref="taxon:1298538"
     regulatory      1..989
                     /regulatory_class="promoter"
                     /gene="pMET3"
     gene            1..989
                     /gene="pMET3"
                     /label=pMET3
     primer_bind     complement(1035..1051)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1059..1075
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1083..1113)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1128..1149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    1776..3500
                     /label=SAT1 marker
                     /note="SAT1 marker"
     rep_origin      complement(3697..4285)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4456..5316)
                     /codon_start=1
                     /gene="bla"
                     /product="Bla"
                     /label=bla
                     /protein_id="AGG53913.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4456..5316)
                     /gene="bla"
                     /label=bla
     promoter        complement(5317..5421)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    complement(5699..6544)
                     /label=RP10 integration sequence
                     /note="RP10 integration sequence"
     primer_bind     6750..6766
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"