LOCUS       Exported                5342 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pAdD26SV(A)-3, complete sequence.
ACCESSION   LT726852
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5342)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Mammalian expression vector pAdD26SV(A)-3"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 729."
                     /db_xref="taxon:1944748"
     CDS             55..1245
                     /codon_start=1
                     /note="unnamed protein product; tetracycline resistance"
                     /protein_id="SJL86676.1"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     rep_origin      complement(1400..1668)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(1450..2038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    3069..3203
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(3129..3225)
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     regulatory      3129..3225
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     CDS             complement(3390..3953)
                     /codon_start=1
                     /note="unnamed protein product; mDhfr"
                     /protein_id="SJL86677.1"
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     misc_feature    complement(4069..4317)
                     /label=hybrid intron late leader/immunoglobulin
                     /note="hybrid intron late leader/immunoglobulin"
     intron          4089..4318
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     regulatory      complement(4318..5101)
                     /regulatory_class="promoter"
                     /note="Adenovirus 2 major late promoter"
     regulatory      5110..5282
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     regulatory      complement(5110..5245)
                     /regulatory_class="promoter"
                     /note="SV40 late promoter"
     promoter        5121..5331
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        5143..5339
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5190..5325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      5239..5314
                     /label=SV40 ori
                     /note="SV40 ori"