LOCUS       Exported                3854 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAC_SalSer914, complete sequence.
ACCESSION   JQ394799
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Anderson JC, Voigt CA, Arkin AP.
  TITLE     Environmental signal integration by a modular AND gate
  JOURNAL   Mol. Syst. Biol. 3, 133 (2007)
  PUBMED    17700541
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Salis HM, Mirsky EA, Voigt CA.
  TITLE     Automated design of synthetic ribosome binding sites to control 
            protein expression
  JOURNAL   Nat. Biotechnol. 27 (10), 946-950 (2009)
  PUBMED    19801975
REFERENCE   3  (bases 1 to 3854)
  AUTHORS   Tamsir A, Tabor JJ, Voigt CA.
  TITLE     Robust multicellular computing using genetically encoded NOR gates 
            and chemical wires
  JOURNAL   Nature 469 (7329), 212-215 (2011)
  PUBMED    21150903
REFERENCE   4  (bases 1 to 3854)
  AUTHORS   Moser F, Voigt CA.
  TITLE     Genetic Circuit Performance under Conditions Relevant for Industrial
            Bioreactors
  JOURNAL   Unpublished
REFERENCE   5  (bases 1 to 3854)
  AUTHORS   Anderson JC, Moser F, Tamsir A, Salis H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JAN-2012) Biological Engineering, MIT, 500 Tech 
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   6  (bases 1 to 3854)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Cloning vector pAC_SalSer914"
                     /mol_type="other DNA"
                     /label=derived from Escherichia coli strain DH10b
                     /note="derived from Escherichia coli strain DH10b"
                     /db_xref="taxon:1175531"
     CDS             complement(218..1120)
                     /codon_start=1
                     /gene="nahR"
                     /product="nahR"
                     /label=nahR
                     /protein_id="AFI26415.1"
                     /translation="MELRDLDLNLLVVFNQLLVDRRVSITAENLGLTQPAVSNALKRLR
                     TSLQDPLFVRTHQGMEPTPYAAHLAEPVTSAMHALRNALQHHESFDPLTSERTFTLAMT
                     DIGEIYFMPRLMDVLAHQAPNCVISTVRDSSMSLMQALQNGTVDLAVGLLPNLQTGFFQ
                     RRLLQNHYVCLCRKDHPVTREPLTLERFCSYGHVRVIAAGTGHGEVDTYMTRVGIRRDI
                     RLEVPHFAAVGHILQRTDLLATVPIRLADCCVEPFGLSALPHPVVLPEIAINMFWHAKY
                     HKDLANIWLRQLMFDLFTD"
     gene            complement(218..1120)
                     /gene="nahR"
                     /label=nahR
     gene            1282..1371
                     /gene="supD"
                     /label=supD
     CDS             1431..1460
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1476..1493
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      1599..1756
                     /regulatory_class="terminator"
                     /note="Trrn"
     terminator      1719..1765
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1943..2602)
                     /codon_start=1
                     /gene="CamR"
                     /product="chloramphenicol acetyltransferase"
                     /label=CamR
                     /protein_id="AFI26414.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(1943..2602)
                     /gene="CamR"
                     /label=CamR
     promoter        complement(2603..2705)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     misc_feature    2968..3808
                     /label=p15A
                     /note="p15A"
     rep_origin      complement(3231..3776)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."