LOCUS       Exported                6369 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pACYC184-ara-xylE, complete sequence.
ACCESSION   KJ170897
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Tsai Y, Liu M-C., Li Z-S., Liaw S-J.
  TITLE     The RNA chaperone Hfq is involved in stress tolerance and virulence 
            in uropathogenic Proteus mirabilis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Tsai Y, Liu M-C., Li Z-S., Liaw S-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2014) Department of Clinical Laboratory Sciences 
            and Medical Biotechnology, College of Medicine, National Taiwan 
            University, 1 Chang-Te St., Taipei 100, Taiwan
REFERENCE   3  (bases 1 to 6369)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Cloning vector pACYC184-ara-xylE"
                     /mol_type="other DNA"
                     /db_xref="taxon:1482868"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      581..1493
                     /label=p15A origin
                     /note="p15A origin"
     rep_origin      complement(848..1393)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        1505..1533
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             complement(1730..2653)
                     /codon_start=1
                     /product="catechol 2,3-dioxygenase"
                     /label=catechol 2,3-dioxygenase
                     /protein_id="AHX26621.1"
                     /translation="MNKGVMRPGHVQLRVLDMSKALEHYVELLGLIEMDRDDQGRVYLK
                     AWTEVDKFSLVLREADEPGMDFMGFKVVDEDALRQLERDLMAYGCAVEQLPAGELNSCG
                     RRVRFQAPSGHHFELYADKEYTGKWGLNDVNPEAWPRDLKGMAAVRFDHALMYGDELPA
                     TYDLFTKVLGFYLAEQVLDENGTRVAQFLSLSTKAHDVAFIHHPEKGRLHHVSFHLETW
                     EDLLRAADLISMTDTSIDIGPTRHGLTHGKTIYFFDPSGNRNEVFCGGDYNYPDHKPVT
                     WTTDQLGKAIFYHDRILNERFMTVLT"
     promoter        complement(2904..3188)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     regulatory      complement(2913..2938)
                     /regulatory_class="promoter"
                     /note="ara"
     CDS             3215..4093
                     /codon_start=1
                     /product="AraC"
                     /label=AraC
                     /note="ara regulator"
                     /protein_id="AHX26622.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     CDS             complement(5929..219)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"