LOCUS       Exported                4895 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pActXYN, complete sequence.
ACCESSION   AY452735
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Vickers CE, Xue GP, Gresshoff PM.
  TITLE     A synthetic xylanase as a novel reporter in plants
  JOURNAL   Plant Cell Rep. 22 (2), 135-140 (2003)
  PUBMED    12845475
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Vickers CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2003) ARC Centre for Integrative Legume Research, 
            The University of Queensland, Room 213, John Hines Building (69), 
            St. Lucia, QLD 4072, Australia
REFERENCE   3  (bases 1 to 4895)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Reporter vector pActXYN"
                     /mol_type="other DNA"
                     /db_xref="taxon:255329"
     misc_feature    267..2274
                     /label=sequence from cloning vector pBR322
                     /note="sequence from cloning vector pBR322"
     promoter        363..467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             468..1328
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampicillin resistance protein; bla"
                     /protein_id="AAR29085.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2345..2367
                     /label=T7 RNA polymerase promoter
                     /note="T7 RNA polymerase promoter"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2426..3338
                     /regulatory_class="promoter"
                     /note="from rice actin gene; Act1"
     promoter        2426..3259
                     /label=Act1 promoter
                     /note="rice actin promoter"
     CDS             3262..3279
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     intron          3339..3792
                     /note="intron 1 from rice actin gene; Act1"
     CDS             3800..4513
                     /codon_start=1
                     /gene="sXynA"
                     /product="Xylanase"
                     /label=sXynA
                     /note="synthetic xylanase"
                     /protein_id="AAR29086.1"
                     /translation="MASNGKKFTVGNGQNQHKGVNDGFSYEIWLDNTGGNGSMTLGSGA
                     TFKAEWNAAVNRGNFLARRGLDFGSQKKATDYDYIGLDYAATYKQTASASGNSRLCVYG
                     WFQNRGLNGVPLVEYYIIEDWVDWVPDAQGKMVTIDGAQYKIFQMDHTGPTINGGSETF
                     KQYFSVRQQKRTSGHITVSDHFKEWAKQGWGIGNLYEVALNAEGWQSSGVADVTLLDVY
                     TTPKGSSPATSAAPR"
     gene            3800..4513
                     /gene="sXynA"
                     /label=sXynA
     regulatory      4535..4872
                     /regulatory_class="terminator"
                     /note="from rbcS gene"
     promoter        complement(4894..17)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    4895
                     /label=SP6 RNA polymerase transcription initiation site
                     /note="SP6 RNA polymerase transcription initiation site"