LOCUS       Exported                6398 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Drosophila transfection vector pact-MCS DNA, complete sequence.
ACCESSION   AB750364
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Ito H, Sato K, Koganezawa M, Ote M, Matsumoto K, Hama C, Yamamoto D.
  TITLE     Fruitless recruits two antagonistic chromatin factors to establish 
            single-neuron sexual dimorphism
  JOURNAL   Cell 149 (6), 1327-1338 (2012)
  PUBMED    22682252
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Ito H, Yamamoto D.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2012) Contact:Hiroki Ito Tohoku University, 
            Graduate School of Life Sciences; 2-1-1 Katahira, Aoba-ku, Sendai, 
            Miyagi 9808577, Japan
REFERENCE   3  (bases 1 to 6398)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Drosophila transfection vector pact-MCS"
                     /lab_host="Escherichia coli DH5 alpha"
                     /mol_type="other DNA"
                     /clone="pact-MCS"
                     /db_xref="taxon:1235437"
     misc_feature    354..2826
                     /label=derived from pUC8 vector
                     /note="derived from pUC8 vector"
     promoter        687..791
                     /gene="bla"
                     /label=AmpR promoter
     CDS             792..1652
                     /codon_start=1
                     /gene="Amp-R"
                     /product="beta-lactamase"
                     /label=Amp-R
                     /protein_id="BAM45098.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            792..1652
                     /gene="Amp-R"
                     /label=Amp-R
     rep_origin      1822..2410
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2698..2719
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2734..2764
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2772..2788
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2796..2812
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2827..5405
                     /regulatory_class="promoter"
                     /note="promoter region of Drosophila Act5C"
     misc_feature    5417..5460
                     /note="multiple cloning site; 
                     EcoRI-BamHI-XbaI-NotI-SacI-KpnI-SalI"
     misc_feature    5461..6398
                     /note="3region containing polyA signal of Drosophila 
                     Act5C"