LOCUS       Exported                3831 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pACS-aadA, complete sequence.
ACCESSION   KP234258
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y, 
            Luzhetskyy A.
  TITLE     From synthetic promoters to new antibiotics: transcriptional 
            activation of silent landomycin A cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Myronovskyi M, Broetz E, Rosenkraenzer B, Manderscheid N, Rebets Y, 
            Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2014) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 3831)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Cloning vector pACS-aadA"
                     /mol_type="other DNA"
                     /db_xref="taxon:1655354"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      446..489
                     /regulatory_class="promoter"
                     /gene="A1-14"
     gene            446..489
                     /gene="A1-14"
                     /label=A1-14
     misc_feature    496..546
                     /label=B-CC
                     /note="B-CC"
     CDS             668..1459
                     /codon_start=1
                     /gene="aadA"
                     /product="Aada"
                     /label=aadA
                     /protein_id="AKJ21148.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            668..1459
                     /gene="aadA"
                     /label=aadA
     misc_feature    1469..1518
                     /label=P-GG
                     /note="P-GG"
     regulatory      1519..1559
                     /regulatory_class="promoter"
                     /gene="P21"
     gene            1519..1559
                     /gene="P21"
                     /label=P21
     primer_bind     complement(1610..1626)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1634..1650
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1658..1688)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1703..1724
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      1997..2616
     rep_origin      complement(2012..2600)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2771..3631)
                     /codon_start=1
                     /gene="bla"
                     /product="Bla"
                     /label=bla
                     /protein_id="AKJ21147.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2771..3631)
                     /gene="bla"
                     /label=bla
     promoter        complement(3632..3736)
                     /gene="bla"
                     /label=AmpR promoter