LOCUS       Exported                9366 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Transfer vector pAcMLF9, complete sequence.
ACCESSION   EF050536
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9366)
  AUTHORS   Harrison RL, Bonning BC.
  TITLE     Use of scorpion neurotoxins to improve the insecticidal activity of 
            Rachiplusia ou multicapsid nucleopolyhedrovirus
  JOURNAL   Biol. Control 17, 191-201 (2000)
REFERENCE   2  (bases 1 to 9366)
  AUTHORS   Harrison RL, Bonning BC.
  TITLE     Use of proteases to improve the insecticidal activity of 
            baculoviruses
  JOURNAL   Biol. Control 20, 199-209 (2001)
REFERENCE   3  (bases 1 to 9366)
  AUTHORS   Harrison RL.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2006) Insect Biocontrol Laboratory, United States 
            Department of Agriculture, Agricultural Research Service, Plant 
            Sciences Institute, 10300 Baltimore Avenue, Beltsville, MD 20705, 
            USA
REFERENCE   4  (bases 1 to 9366)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9366
                     /organism="Transfer vector pAcMLF9"
                     /focus
                     /mol_type="other DNA"
                     /note="pAcMLF9 is a transfer vector for making recombinant 
                     Autographa californica multiple nucleopolyhedrovirus 
                     (AcMNPV) that is occlusion-positive and expresses foreign 
                     genes from the p6.9 promoter"
                     /db_xref="taxon:410857"
     source          1..2099
                     /organism="Autographa californica MNPV"
                     /mol_type="other DNA"
                     /clone="C6"
                     /db_xref="taxon:307456"
     source          2562..2893
                     /organism="Autographa californica MNPV"
                     /mol_type="other DNA"
                     /clone="C6"
                     /label=includes upstream region of the p6.9 gene
                     /note="includes upstream region of the p6.9 gene"
                     /db_xref="taxon:307456"
     source          2920..6251
                     /organism="Autographa californica MNPV"
                     /mol_type="other DNA"
                     /clone="C6"
                     /label=includes polyhedrin promoter and coding sequence
                     /note="includes polyhedrin promoter and coding sequence"
                     /db_xref="taxon:307456"
     misc_recomb     1269..2096
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             1269..1393
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(1431..2036)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     regulatory      complement(2102..2556)
                     /regulatory_class="polyA_signal_sequence"
                     /note="derived from vector pCH110; includes lacZ stop codon
                     and the SV40 early region polyadenylation signal and 
                     polyadenylation site"
     polyA_signal    2102..2236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(2563..2887)
                     /gene="basic protein gene from Autographa californica"
                     /label=basic protein promoter
                     /note="baculovirus basic protein promoter, which allows 
                     expression during the late instead of very late phase of 
                     viral infection"
     promoter        2920..3011
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_recomb     3662..5120
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3UTR           3750..4122
                     /label=polyhedrin 3 UTR
                     /note="3 UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(3779..5120)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     misc_feature    6252..9366
                     /label=derived from pUC118
                     /note="derived from pUC118"
     primer_bind     complement(6260..6276)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6489..6944
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7226..7330
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7331..8191
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8362..8950
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9238..9259
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9274..9304
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9312..9328
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9336..9352
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"