LOCUS       Exported                8796 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pACCMVpLpA, complete sequence.
ACCESSION   LT727333
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8796)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8796)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8796
                     /organism="Mammalian expression vector pACCMVpLpA"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 4235."
                     /db_xref="taxon:1944745"
     rep_origin      1..454
                     /label=Ad5 ori
                     /note="Ad5 ori"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     191..341
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     regulatory      673..1256
                     /regulatory_class="promoter"
                     /note="hCMV-IE promoter and enhancer"
     promoter        1053..1256
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    1287..1343
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             1346..1420
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small 
                     t-antigen"
                     /protein_id="SJL88136.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    1421..1487
                     /label=intron of SV40 small t-antigen
                     /note="intron of SV40 small t-antigen"
     intron          1422..1487
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     regulatory      complement(1625..1721)
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     regulatory      1625..1721
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     polyA_signal    1647..1781
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    1795..4697
                     /label=Ad5 flanking np3334-6236
                     /note="Ad5 flanking np3334-6236"
     rep_origin      complement(6778..7180)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(6962..7550)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7721..8581)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL88137.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8582..8686)
                     /gene="bla"
                     /label=AmpR promoter