LOCUS       Exported                8245 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAc5.1B_FN_su(ur), complete sequence.
ACCESSION   KX357892
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN, 
            Belyaeva ES, Belyakin SN.
  TITLE     Repressive Histone Marks Renewal Impedes Replication Fork 
            Progression and Contributes To Late Replication in Drosophila
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8245)
  AUTHORS   Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN, 
            Belyaeva ES, Belyakin SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2016) Genomics lab, IMCB SD RAS, Lavrentjeva ave. 
            8/2, Novosibirsk, NSO 630090, Russia
REFERENCE   3  (bases 1 to 8245)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="Cloning vector pAc5.1B_FN_su(ur)"
                     /mol_type="other DNA"
                     /db_xref="taxon:1943577"
     regulatory      46..2568
                     /regulatory_class="promoter"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2635..5646
                     /codon_start=1
                     /product="SU(UR)+FLAG"
                     /label=SU
                     /note="Su(UR) gene cDNA with 3 FLAG sequences"
                     /protein_id="AQA27272.1"
                     /translation="MYHFVSEQTPEVRLTDEALVTSHVTQYLKSFQLDAVRFVYDRLAK
                     REFCILNDESGLGKVATVAALLSALPPAKKTLVVLQNDEQLLTGWRFHLDTLTDLQVYI
                     IQGVQDTTDSPHSVYLAKWSQLRSIGDLSRLKFDYIMVDNRGHSLNNSFCTSMLLKQFE
                     GRVNVLISSVDVTSDVRLLYNVLRLGGRLEHQYKSFASFDRKFHLPDPKEVFSKRIDLE
                     EYYKQRGFLSEYIKDFRLRRFRHQFDKSLPLVAPEQYKHNLNLWLASKNSQSTISGSDV
                     CSTIASIDNNPAQQNKTGLFEETDRLSEHSVDDVAMSPLIFEYSESDDEPLTVEPDADQ
                     NPVLVVSSDDCEIVTPPSTPQNRTPSLNESPRTKSKKKFSKKTSPRKKADLTDSEEDDE
                     ATDNMPPRKRTRAATVHLTPKTRRLNVRILRVSLDTLSTPPPSRTTTAIVTPKTEPTAR
                     RKNLKKRTVSPVDVGRPATRGMQRLTRSAETKINSKYLKHRSLDDVKRSFPRRVKLEGN
                     QTPRSSKQIVKQEPKSKVGQEKKQKTVDVPAQGTAKRKPGRPRKCQTKTEDLGKTKTKP
                     NSKHLPPTPQVLSGSSLSSEYMQCAQRIPDNLDAIESPAFRVPFTPQQTPMLLTLPSTH
                     NLLNDSEVVSIPLYKDPVETVVINSSHDECSPQDPSQSRRTKALKRKRKPVTSVNSSFG
                     GGLGLPPAKRSANKSPDLFSISSEHSQIPLAQPRPSSPFEGFKIFGSEVKQFQQQLAKV
                     NISVPKKKRDRSCLDILEQMFEPRQQQSAKTSPKVLPTLPLTQKDDAESTITQRRRTLL
                     EDDFFEITNNGQFGSRMRLNSSGEVSPVQPDQQSVRPSQANKITNYLIGSGITQERTQP
                     SNGNRNSILASLRKSPKSPKQGAKSTQATKLTRWFGSVFGGGASQTSSVESVSAPSTPV
                     NSSTSAAACQTRSARSGGASGPTKRKRLELFKSAAMAAGSDVGSTSMDYKDHDGDYKDH
                     DIDYKDDDDKLVLA"
     regulatory      5528..5537
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             5566..5631
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    5671..5805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(6427..7015)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7186..8046)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8047..8151)
                     /gene="bla"
                     /label=AmpR promoter