LOCUS       Exported                5368 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAc1, complete sequence.
ACCESSION   EU446025
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Lindner AB, Madden R, Demarez A, Stewart EJ, Taddei F.
  TITLE     Asymmetric segregation of protein aggregates is associated with 
            cellular aging and rejuvenation
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 105 (8), 3076-3081 (2008)
  PUBMED    18287048
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Lindner AB.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2008) U571, INSERM, 156 rue de Vaugirard, Paris 
            75015, France
REFERENCE   3  (bases 1 to 5368)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Cloning vector pAc1"
                     /mol_type="other DNA"
                     /db_xref="taxon:505188"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             201..1061
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2510..3658
                     /codon_start=1
                     /product="inclusion body protein A - yellow fluorescent 
                     protein fusion"
                     /label=inclusion body protein A - yellow fluorescent p
                     /note="small heat shock protein -fluorescent protein 
                     fusion"
                     /protein_id="ACA30203.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDEN
                     HYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLAEN
                     IHVRGANLVNGLLYIDLERVIPEAKKPRRIEINDPPVATMASKGEELFTGVVPILVELD
                     GDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHM
                     KRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNIL
                     GHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLP
                     DNHYLSYQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK"
     protein_bind    complement(3695..3742)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        complement(4417..4519)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(4625..4658)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(4974..4990)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"