LOCUS       Exported               11516 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pABCc, complete sequence.
ACCESSION   KY031726
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11516)
  AUTHORS   Doehlemann J, Wagner M.
  TITLE     A family of single copy repABC-type shuttle vectors stably 
            maintained in the alpha-proteobacterium Sinorhizobium meliloti
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11516)
  AUTHORS   Doehlemann J, Wagner M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2016) LOEWE-Zentrum fuer Synthetische 
            Mikrobiologie, Philipps-Universitaet Marburg, Hans-Meerwein-Strasse,
            Marburg, Hesse 35043, Germany
REFERENCE   3  (bases 1 to 11516)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11516
                     /organism="Cloning vector pABCc"
                     /mol_type="other DNA"
                     /db_xref="taxon:1928855"
     misc_feature    1..7857
                     /label=oriVSm part (oriV_p42d)
                     /note="oriVSm part (oriV_p42d)"
     misc_feature    7858..8417
                     /label=synTer-MCS part (synTer3)
                     /note="synTer-MCS part (synTer3)"
     terminator      7880..7907
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      8045..8072
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      8209..8295
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_feature    8379..8408
                     /label=MCS
                     /note="MCS"
     misc_feature    8418..10641
                     /label=oriVEc part (oriV_pSC101*)
                     /note="oriVEc part (oriV_pSC101*)"
     CDS             9167..10117
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     misc_feature    10642..11516
                     /label=AR part (kanamycin resistance cassette)
                     /note="AR part (kanamycin resistance cassette)"
     CDS             complement(10650..11444)
                     /codon_start=1
                     /gene="aph(3)-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"