LOCUS       Exported                7406 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAB707, complete sequence.
ACCESSION   JN005928
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Murakami T, Burian J, Yanai K, Bibb MJ, Thompson CJ.
  TITLE     A system for the targeted amplification of bacterial gene clusters 
            multiplies antibiotic yield in Streptomyces coelicolor
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 108 (38), 16020-16025 (2011)
  PUBMED    21903924
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   Murakami T, Burian J IV, Yanai K, Bibb MJ, Thompson CJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2011) Microbiology and Immunology, University of 
            British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, 
            Canada
REFERENCE   3  (bases 1 to 7406)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Cloning vector pAB707"
                     /mol_type="other DNA"
                     /db_xref="taxon:1095419"
     CDS             complement(107..127)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    173..804
                     /label=derived from Streptomyces kanamyceticus
                     /note="derived from Streptomyces kanamyceticus"
     misc_recomb     503..527
                     /label=recombination site RsA
                     /note="recombination site RsA"
     misc_recomb     complement(1002..1035)
                     /label=FRT site
                     /note="FRT site"
     protein_bind    1002..1035
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     oriT            1042..1151
                     /note="incP origin of transfer"
     oriT            complement(1044..1153)
                     /direction=LEFT
                     /rpt_family="RK2"
     CDS             1467..2267
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILHDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     gene            1494..2275
                     /gene="aac(3)IV"
                     /label=aac(3)IV
     CDS             1494..2270
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="apramycin resistance protein"
                     /label=aac(3)IV
                     /note="Aac(3)IV"
                     /protein_id="AEQ39732.1"
                     /translation="MQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRPLEDGPLGLI
                     EALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNVKRSAHPFAF
                     AAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLHLAELMAKVP
                     YGVPRHCTILHDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGHAFARLIRSR
                     DIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     protein_bind    2277..2323
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_recomb     complement(2290..2323)
                     /label=FRT site
                     /note="FRT site"
     misc_feature    2343..4428
                     /label=derived from Streptomyces kanamyceticus
                     /note="derived from Streptomyces kanamyceticus"
     misc_recomb     4039..4066
                     /label=recombination site RsB
                     /note="recombination site RsB"
     promoter        complement(4712..4730)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4845..4949
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4950..5810
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             5145..5810
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="Bla; ampicillin resistance"
                     /protein_id="AEQ39733.1"
                     /translation="MMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKH
                     LTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNE
                     AIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWF
                     IADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     gene            5145..5810
                     /gene="bla"
                     /label=bla
     rep_origin      5965..6584
     rep_origin      5981..6569
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    6954..7088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"