LOCUS       Exported                6566 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pAB26-EO6, complete sequence.
ACCESSION   DQ115383
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Dennehy ME, Bourn W, Steele AD, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice 
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6566)
  AUTHORS   Dennehy ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2005) Medical Virology, University of Cape Town, 
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 6566)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Shuttle vector pAB26-EO6"
                     /mol_type="other DNA"
                     /note="Mycobacterium-E. coli shuttle vector; murine EO 
                     rotavirus VP6 of GenBank Accession Number AY947543 cloned 
                     into BamH1-Hind111 sites of Mycobacterium-E. coli shuttle 
                     vector pAB26"
                     /db_xref="taxon:336701"
     misc_feature    7..1219
                     /note="APH1 region of Tn903 encoding 
                     aminoglycoside-3-phosphotransferase, conferring kanamycin 
                     resistance"
     CDS             121..936
                     /codon_start=1
                     /gene="aph(3)-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1224..2010
                     /label=OriE region derived from pUC19
                     /note="OriE region derived from pUC19"
     rep_origin      1268..1856
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2029..3927
                     /label=Mycobacterial OriM region derived from pAL5000
                     /note="Mycobacterial OriM region derived from pAL5000"
     regulatory      3957..4197
                     /regulatory_class="promoter"
                     /note="derived from Mycobacterium bovis hsp60 promoter of 
                     GenBank Accession Number M17705"
     misc_feature    4217..5224
                     /label=similar to Mycobacterium bovis alpha antigen
                     /note="similar to Mycobacterium bovis alpha antigen"
     regulatory      4240..4244
                     /regulatory_class="ribosome_binding_site"
                     /note="alpha antigen Shine-Dalgarno sequence"
     CDS             4254..6428
                     /codon_start=1
                     /product="alpha antigen/VP6 chimera"
                     /label=alpha antigen/VP6 chimera
                     /note="40 amino acid signal sequence, 2 inserted amino 
                     acids and 324 amino acids of mature protein of 
                     Mycobacterium bovis alpha antigen linked to 397 amino acids
                     of murine EO rotavirus VP6"
                     /protein_id="AAZ16501.1"
                     /translation="MTDVSRKIRAWGRRLMIGTAAAVVLPGLVGLAGGAATAGAFSRPG
                     LPVEYLQVPSPSMGRDIKVQFQSGGNNSPAVYLLDGLRAQDDYNGWDINTPAFEWYYQS
                     GLSIVMPVGGQSSFYSDWYSPACGKAGCQTYKWETLLTSELPQWLSANRAVKPTGSAAI
                     GLSMAGSSAMILAAYHPQQFIYAGSLSALLDPSQGMGPSLIGLAMGDAGGYKAADMWGP
                     SSDPAWERNDPTQQIPKLVANNTRLWVYCGNGTPNELGGANIPAEFLENFVRSSNLKFQ
                     DAYKPAGGHNAVFNFPPNGTHSWEYWGAQLNAMKGDLQSSLGADPKMDVLYSISRTLKD
                     ARDKIVEGTLYSNVSDLIQQFNQMLVTMNGNEFQTGGIGNLPLRNWNFDFGLLGTTLLN
                     LDANYVESARTTIDYFVDFIDNVCMDEMVRESQRNGIAPQSDALRKLSGVKFRRINFNN
                     SSEYIENWNLQNRRQRTGFTFHKPNIFPYSASFTLNRSQPQHDNLMGTMWLNAGSEIQV
                     AGFDYSCAINAPANIQQFEHIVQLRRVLTTATITLLPDAERFSFPRVINSADGATTWYF
                     NPVILRPNNVEVEFLLNGQIINTYQARFGTIVARNFDTIRLSFQLMRPPNMTPAVAALF
                     PNAQPFEHHATVGLTLRIDSAICESVLADASETMLANVTSVRQEYAIPVGPVFPPGMNW
                     TDLITNYSPSREDNLQRVFTVASIRSMLVK"
     misc_feature    4852..4857
                     /label=six inserted nucleotides
                     /note="six inserted nucleotides"
     misc_feature    5235..6428
                     /note="derived from murine EO rotavirus VP6 of GenBank 
                     Accession Number AY947543"
     terminator      6484..6530
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      6487..6530
                     /regulatory_class="terminator"
                     /note="rrnBT1"