LOCUS       Exported                7187 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  SiRNA vector pAAV9(5)-CMV-EGFP-CytB-AS-ohneNot, complete sequence.
ACCESSION   AY640632
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Michel U, Malik I, Ebert S, Bahr M, Kugler S.
  TITLE     Long-term in vivo and in vitro AAV-2-mediated RNA interference in 
            rat retinal ganglion cells and cultured primary neurons
  JOURNAL   Biochem. Biophys. Res. Commun. 326 (2), 307-312 (2005)
  PUBMED    15582578
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   Michel U, Malik I, Kuegler S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2004) Neurology, University of Goettingen, Waldweg
            33, Goettingen 37073, Germany
REFERENCE   3  (bases 1 to 7187)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="siRNA vector pAAV9(5)-CMV-EGFP-CytB-AS-ohneNot"
                     /mol_type="other DNA"
                     /db_xref="taxon:297634"
     repeat_region   1..141
                     /label=left ITR
                     /rpt_family="inverted"
                     /note="left ITR"
     regulatory      complement(38..193)
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40-pA; SV40 polyadenylation sequence"
     polyA_signal    194..328
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          complement(198..341)
                     /note="SV40 chimeric intron derived from pCI-NEO"
     intron          344..477
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(489..1208)
                     /codon_start=1
                     /gene="EGFP"
                     /product="enhanced green fluorescent protein"
                     /label=EGFP
                     /note="derived from Clontechs pEGFP-N1"
                     /protein_id="AAV73966.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            complement(489..1208)
                     /gene="EGFP"
                     /label=EGFP
     regulatory      1205..1214
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    complement(1227..1246)
                     /note="MCS; multiple cloning site"
     regulatory      complement(1257..1779)
                     /regulatory_class="promoter"
                     /note="MCMV promoter; mcmv promoter (-491 to +36)"
     primer_bind     1779..1795
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1815..2030
                     /regulatory_class="promoter"
                     /note="disabled human H1 RNA promoter"
     promoter        1815..2029
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     misc_feature    2031..2162
                     /note="Cytb-AS; partial human cytochrome B antisense 
                     sequence"
     gene            complement(2167..4430)
                     /gene="9(5)"
                     /label=9(5)
                     /note="partial sequence from the non-coding porcine RNA UM 
                     9(5)"
     repeat_region   4450..4590
                     /label=right ITR
                     /rpt_family="inverted"
                     /note="right ITR"
     rep_origin      4665..5120
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5402..5506
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5507..6367
                     /codon_start=1
                     /gene="Amp"
                     /product="beta-lactamase"
                     /label=Amp
                     /note="ampicillin resistance"
                     /protein_id="AAV73967.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            5507..6367
                     /gene="Amp"
                     /label=Amp
     rep_origin      6538..7126
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"