LOCUS       Exported                5230 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAAV-ISceI.AO3, complete sequence.
ACCESSION   EU022316
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Inagaki K, Lewis SM, Wu X, Ma C, Munroe DJ, Fuess S, Storm TA, Kay 
            MA, Nakai H.
  TITLE     DNA palindromes with a modest arm length of greater, similar 20 base
            pairs are a significant target for recombinant adeno-associated 
            virus vector integration in the liver, muscles, and heart in mice
  JOURNAL   J. Virol. 81 (20), 11290-11303 (2007)
  PUBMED    17686840
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Nakai H, Inagaki K.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2007) Department of Molecular Genetics and 
            Biochemistry, University of Pittsburgh School of Medicine, W1244 BST
            200 Lothrop Street, Pittsburgh, PA 15261, USA
REFERENCE   3  (bases 1 to 5230)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Cloning vector pAAV-ISceI.AO3"
                     /mol_type="other DNA"
                     /db_xref="taxon:474140"
     CDS             640..660
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             664..684
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             688..708
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    741..758
                     /label=I-Sce I recognition site
                     /note="I-Sce I recognition site"
     regulatory      764..817
                     /regulatory_class="promoter"
                     /note="transposon Tn3 promoter from GenBank Accession 
                     Number V00613"
     CDS             818..1678
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="confers ampicillin resistance"
                     /label=bla
                     /protein_id="ABV69054.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            818..1678
                     /gene="bla"
                     /label=bla
     rep_origin      1826..2469
                     /label=pUC
                     /note="pUC"
     rep_origin      1849..2437
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     repeat_region   2512..2718
                     /label=murine leukemia virus (MLV) LTR from NC_001501
                     /note="murine leukemia virus (MLV) LTR from NC_001501"
     repeat_region   2780..2920
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2780..2909
                     /label=adeno-associated virus serotype 2
                     /note="adeno-associated virus serotype 2"
     rep_origin      2995..3450
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        complement(3862..3890)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      4440..5028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     repeat_region   5090..5230
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5101..5230
                     /label=adeno-associated virus serotype 2
                     /note="adeno-associated virus serotype 2"