LOCUS       Exported                7278 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAAV-hSyn-mCherry-hSyn-9(5), complete sequence.
ACCESSION   KT345943
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Michel U, Muller B, Koch JC, Zhang J-N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2015) Neurology, University of Goettingen, Waldweg
            33, Goettingen, Niedersachsen 37073, Germany
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Cloning vector pAAV-hSyn-mCherry-hSyn-9(5)"
                     /mol_type="other DNA"
                     /db_xref="taxon:1776678"
     intron          17..157
                     /note="beta-globin-IgG-chimeric intron"
     intron          20..153
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     regulatory      187..656
                     /regulatory_class="promoter"
                     /note="human synapsin promoter (hSyn)"
     promoter        complement(193..640)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     complement(676..692)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    737..879
                     /label=ITR
                     /note="ITR"
     rep_origin      complement(1141..1729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      1328..1553
     CDS             complement(1900..2760)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="ALX18067.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2761..2865)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3197..3652)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      3207..3666
                     /label=f1 (+) ORI
                     /note="f1 (+) ORI"
     misc_feature    3753..3896
                     /label=ITR
                     /note="ITR"
     polyA_signal    3906..4113
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    3918..4128
                     /label=bGH pA
                     /note="bGH pA"
     misc_feature    4152..4745
                     /label=WPRE
                     /note="WPRE"
     misc_feature    4155..4743
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4261..4272
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      5731..6210
                     /regulatory_class="promoter"
                     /note="human synapsin promoter (hSyn)"
     promoter        complement(5737..6184)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    6237..6397
                     /label=SV40-pA
                     /note="SV40-pA"
     polyA_signal    6250..6384
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          6397..6541
                     /note="chimeric intron derived from pCI-NEO"
     intron          6400..6533
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    complement(6549..7259)
                     /label=similar to mcherry
                     /note="similar to mcherry"
     CDS             complement(6549..7259)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"