LOCUS       Exported                7495 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAAV-hSyn-CRMP2-hSyn-mCherry, complete sequence.
ACCESSION   KT345944
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Michel U, Muller B, Koch JC, Zhang J-N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2015) Neurology, University of Goettingen, Waldweg
            33, Goettingen, Niedersachsen 37073, Germany
REFERENCE   2  (bases 1 to 7495)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Cloning vector pAAV-hSyn-CRMP2-hSyn-mCherry"
                     /mol_type="other DNA"
                     /db_xref="taxon:1776677"
     misc_feature    48..190
                     /label=ITR
                     /note="ITR"
     rep_origin      complement(452..1040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      639..864
     CDS             complement(1211..2071)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="ALX18068.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2072..2176)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2508..2963)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    2518..2977
                     /label=f1 (+) ORI
                     /note="f1 (+) ORI"
     misc_feature    3064..3207
                     /label=ITR
                     /note="ITR"
     polyA_signal    3217..3424
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    3229..3439
                     /label=bGH pA
                     /note="bGH pA"
     CDS             complement(3507..5225)
                     /codon_start=1
                     /gene="CRMP2"
                     /product="collapsin response mediator protein-2"
                     /label=CRMP2
                     /note="derived from Homo sapiens"
                     /protein_id="ALX18069.1"
                     /translation="MSYQGKKNIPRITSDRLLIKGGKIVNDDQSFYADIYMEDGLIKQI
                     GENLIVPGGVKTIEAHSRMVIPGGIDVHTRFQMPDQGMTSADDFFQGTKAALAGGTTMI
                     IDHVVPEPGTSLLAAFDQWREWADSKSCCDYSLHVDISEWHKGIQEEMEALVKDHGVNS
                     FLVYMAFKDRFQLTDCQIYEVLSVIRDIGAIAQVHAENGDIIAEEQQRILDLGITGPEG
                     HVLSRPEEVEAEAVNRAITIANQTNCPLYITKVMSKSSAEVIAQARKKGTVVYGEPITA
                     SLGTDGSHYWSKNWAKAAAFVTSPPLSPDPTTPDFLNSLLSCGDLQVTGSAHCTFNTAQ
                     KAVGKDNFTLIPEGTNGTEERMSVIWDKAVVTGKMDENQFVAVTSTNAAKVFNLYPRKG
                     RIAVGSDADLVIWDPDSVKTISAKTHNSSLEYNIFEGMECRGSPLVVISQGKIVLEDGT
                     LHVTEGSGRYIPRKPFPDFVYKRIKARSRLAELRGVPRGLYDGPVCEVSVTPKTVTPAS
                     SAKTSPAKQQAPPVRNLHQSGFSLSGAQIDDNIPRRTTQRIVAPPGGRANITSLG"
     gene            complement(3507..5225)
                     /gene="CRMP2"
                     /label=CRMP2
     regulatory      5265..5744
                     /regulatory_class="promoter"
                     /note="human synapsin promoter (hSyn)"
     promoter        complement(5271..5718)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    5771..5929
                     /label=SV40-pA
                     /note="SV40-pA"
     polyA_signal    5782..5916
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          5929..6073
                     /note="chimeric intron derived from pCI-NEO"
     intron          5932..6065
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    6081..6791
                     /label=similar to mcherry
                     /note="similar to mcherry"
     CDS             complement(6081..6791)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     intron          6823..6963
                     /note="beta-globin-IgG-chimeric intron"
     intron          6826..6959
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     regulatory      6993..7462
                     /regulatory_class="promoter"
                     /note="human synapsin promoter (hSyn)"
     promoter        complement(6999..7446)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     complement(7482..3)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"