LOCUS       Exported                6772 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAAV-EF1alpha-hFAH.AOS2, complete sequence.
ACCESSION   EF203084
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Nakai H, Wu X, Fuess S, Storm TA, Munroe D, Montini E, Burgess SM, 
            Grompe M, Kay MA.
  TITLE     Large-scale molecular characterization of adeno-associated virus 
            vector integration in mouse liver
  JOURNAL   J. Virol. 79 (6), 3606-3614 (2005)
  PUBMED    15731255
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Nakai H, Wu X, Fuess S, Storm TA, Munroe D, Montini E, Burgess SM, 
            Grompe M, Kay MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JAN-2007) Department of Molecular Genetics and 
            Biochemistry, University of Pittsburgh School of Medicine, W1244 BST
            200 Lothrop Street, Pittsburgh, PA 15261, USA
REFERENCE   3  (bases 1 to 6772)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Cloning vector pAAV-EF1alpha-hFAH.AOS2"
                     /mol_type="other DNA"
                     /db_xref="taxon:420886"
     regulatory      74..1205
                     /regulatory_class="promoter"
                     /note="Homo sapiens eukaryotic translation elongation 
                     factor 1 alpha promoter"
     intron          233..1175
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             1243..2502
                     /codon_start=1
                     /gene="FAH"
                     /product="fumarylacetoacetate hydrolase"
                     /label=FAH
                     /note="derived form Homo sapiens"
                     /protein_id="ABM91085.1"
                     /translation="MSFIPVAEDSDFPIHNLPYGVFSTRGDPRPRIGVAIGDQILDLSI
                     IKHLFTGPVLSKHQDVFNQPTLNSFMGLGQAAWKEARVFLQNLLSVSQARLRDDTELRK
                     CAFISQASATMHLPATIGDYTDFYSSRQHATNVGIMFRDKENALMPNWLHLPVGYHGRA
                     SSVVVSGTPIRRPMGQMKPDDSKPPVYGACKLLDMELEMAFFVGPGNRLGEPIPISKAH
                     EHIFGMVLMNDWSARDIQKWEYVPLGPFLGKSFGTTVSPWVVPMDALMPFAVPNPKQDP
                     RPLPYLCHDEPYTFDINLSVNLKGEGMSQAATICKSNFKYMYWTMLQQLTHHSVNGCNL
                     RPGDLLASGTISGPEPENFGSMLELSWKGTKPIDLGNGQTRKFLLDGDEVIITGYCQGD
                     GYRIGFGQCAGKVLPALLPS"
     gene            1243..2502
                     /gene="FAH"
                     /label=FAH
     regulatory      2625..2834
                     /regulatory_class="polyA_signal_sequence"
                     /note="poly A signal from Homo sapiens beta globin"
     promoter        2878..2982
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2983..3843
                     /codon_start=1
                     /gene="bla"
                     /product="beta lactamase"
                     /label=bla
                     /note="confers ampicillin resistance"
                     /protein_id="ABM91086.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            2983..3843
                     /gene="bla"
                     /label=bla
     rep_origin      3991..4634
                     /label=pUC plasmid replication origin
                     /note="pUC plasmid replication origin"
     rep_origin      4014..4602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     repeat_region   4734..4863
                     /note="adeno-associated virus serotype 2 inverted terminal 
                     repeat"
     repeat_region   6643..6772
                     /note="adeno-associated virus serotype 2 inverted terminal 
                     repeat"