LOCUS       Exported                6994 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAAV-9(5)hSyn-DsRed2N1-NRSEdsRNA, complete sequence.
ACCESSION   HQ416703
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Koch JC, Barski E, Lingor P, Bahr M, Michel U.
  TITLE     Plasmids containing NRSE/RE1 sites enhance neurite outgrowth of 
            retinal ganglion cells via sequestration of REST independent of NRSE
            dsRNA expression
  JOURNAL   FEBS J. 278 (18), 3472-3483 (2011)
  PUBMED    21790997
REFERENCE   2  (bases 1 to 6994)
  AUTHORS   Koch JC, Michel U.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2010) Neurology, University Medicine Goettingen, 
            Robert-Koch-Str. 40, Goettingen, Niedersachsen 37075, Germany
REFERENCE   3  (bases 1 to 6994)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Cloning vector
                     pAAV-9(5)hSyn-DsRed2N1-NRSEdsRNA"
                     /mol_type="other DNA"
                     /note="derived from the sequence of INSD accession 
                     AY640633"
                     /db_xref="taxon:1007531"
     misc_feature    1..141
                     /label=left ITR from AAV2
                     /note="left ITR from AAV2"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     polyA_signal    194..328
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          complement(341..481)
                     /note="chimeric intron"
     intron          344..477
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    complement(491..1175)
                     /gene="DsRed2N1"
                     /label=similar to DsRed
                     /note="similar to DsRed"
     gene            complement(491..1175)
                     /gene="DsRed2N1"
                     /label=DsRed2N1
     CDS             complement(491..1168)
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     regulatory      1165..1174
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     regulatory      1205..1674
                     /regulatory_class="promoter"
                     /note="human synapsin promoter (hSyn)"
     promoter        complement(1211..1658)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      1674..1685
                     /regulatory_class="promoter"
                     /note="human H1 promoter (H1)"
     promoter        1686..1900
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     misc_feature    1902..1969
                     /label=NRSE double-stranded RNA
                     /note="NRSE double-stranded RNA"
     misc_feature    1974..4237
                     /label=partial sequence of porcine non-coding RNA 9(5)
                     /note="partial sequence of porcine non-coding RNA 9(5)"
     misc_feature    4257..4397
                     /label=right ITR from AAV2
                     /note="right ITR from AAV2"
     repeat_region   4257..4397
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4472..4927
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5209..5313
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5314..6174
                     /codon_start=1
                     /gene="amp"
                     /product="ampicillin resistance protein"
                     /label=amp
                     /protein_id="AEJ82523.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            5314..6174
                     /gene="amp"
                     /label=amp
     rep_origin      6345..6933
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"