LOCUS       Exported                7187 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAAV-9(5)-CMV-EGFP-CytbAS, complete sequence.
ACCESSION   KT343252
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Michel U, Muller B, Koch JC, Zhang J-N.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2015) Neurology, University of Goettingen, Waldweg
            33, Goettingen, Niedersachsen 37073, Germany
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Cloning vector pAAV-9(5)-CMV-EGFP-CytbAS"
                     /mol_type="other DNA"
                     /db_xref="taxon:1769783"
     misc_feature    27..187
                     /label=SV40-pA
                     /note="SV40-pA"
     polyA_signal    40..174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          187..331
                     /note="SV40 polyadenylation sequence"
     intron          190..323
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             335..1054
                     /codon_start=1
                     /gene="EGFP"
                     /product="enhanced green fluorescent protein"
                     /label=EGFP
                     /protein_id="ALS87664.1"
                     /translation="LLVQLVHAESDPGGGHELQQDHVIALLVGVFAQGGLGAQVVVVGQ
                     QHGAVADGGVLLVVVGELHAAVLDVVADLEVHLDAVLLLVGHDIDVVAVVVVLQLVPQD
                     VAVLLEVDALQLDAVHQGVALELHLGAGLVVAVVLEEDGALLDVAFGHGGLEEVVLLHV
                     VGVAAEALHAVGQGGHEGGPGHGQLAGGADELQGQLAVGGIALALAGHAELVAVYVAVQ
                     LDQDGHHPGEQLLALAHH"
     gene            335..1054
                     /gene="EGFP"
                     /label=EGFP
     regulatory      1051..1060
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     regulatory      1103..1625
                     /regulatory_class="promoter"
                     /note="mCMV promoter"
     primer_bind     1625..1641
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1661..1876
                     /regulatory_class="promoter"
                     /note="H1 RNA promoter"
     promoter        1661..1875
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     misc_feature    4296..4436
                     /label=right ITR
                     /note="right ITR"
     repeat_region   4296..4436
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4511..4966
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5248..5352
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5353..6213
                     /codon_start=1
                     /gene="Amp"
                     /product="ampicillin resistance protein"
                     /label=Amp
                     /protein_id="ALS87665.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            5353..6213
                     /gene="Amp"
                     /label=Amp
     rep_origin      6384..6972
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7034..7174
                     /label=left ITR
                     /note="left ITR"
     repeat_region   7034..7174
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"