LOCUS       Exported                9583 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Sequencing vector pAA3.7X, complete sequence.
ACCESSION   M11320
VERSION     .
KEYWORDS    complete genome.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9583)
  AUTHORS   Ahmed A.
  TITLE     A rapid procedure for DNA sequencing using transposon-promoted 
            deletions in Escherichia coli
  JOURNAL   Gene 39 (2-3), 305-310 (1985)
  PUBMED    3005115
REFERENCE   2  (bases 1 to 9583)
  AUTHORS   Ahmed A.
  TITLE     Sequencing with transposon-promoted deletions: Use of 
            transposon-promoted deletions in DNA sequence analysis
  JOURNAL   (in) Wu,R. and Grossman,L. (Eds.); METHODS IN ENZYMOLOGY, 
            RECOMBINANT DNA, PART E; Academic Press, New York (1986)
REFERENCE   3  (bases 1 to 9583)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     [2]  revises [1].
            Sequence in computer readable form and reprints for [1],[(in) Wu,R. 
            and Grossman,L., (Eds.);Methods in Enzymology, Recombinant DNA, part
            ] and [2]
            kindly provided by A.Ahmed, 31-MAR-1986.
            [1] describes a new quick sequencing method, which involves the 
            subdivision of the DNA fragment to be sequenced into many 
            overlapping segments by a series of deletions which start from a 
            fixed site.  Since the different segments retain their natural 
            connectedness, the complete sequence is easily assembled from 
            overlaps that occur between the sequences of adjacent deletions.  A 
            simple and rapid method for the isolation of transposon-promoted 
            deletions and their use in sequencing long DNA fragments was 
            developed.
FEATURES             Location/Qualifiers
     source          1..9583
                     /organism="Sequencing vector pAA3.7X"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:2517"
     CDS             2927..4117
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     CDS             4758..4949
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    5051..5191
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5377..5965)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6136..6996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6997..7101)
                     /gene="bla"
                     /label=AmpR promoter
     mobile_element  7122..7889
                     /mobile_element_type="insertion sequence:IS1"
                     /note="prokaryotic transposable element"
     promoter        8010..8112
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             8113..8772
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"