LOCUS       Exported                5143 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Phagemid cloning vector pA2, complete sequence.
ACCESSION   EU481505
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Fagerlund A, Myrset AH, Kulseth MA.
  TITLE     Construction and characterization of a 9-mer phage display 
            pVIII-library with regulated peptide density
  JOURNAL   Appl. Microbiol. Biotechnol. 80 (5), 925-936 (2008)
  PUBMED    18716770
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Fagerlund A, Myrset AH, Kulseth MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2008) GE Healthcare Medical Diagnostics, P.O. Box 
            4220 Nydalen, Oslo N-0401, Norway
REFERENCE   3  (bases 1 to 5143)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="phagemid cloning vector pA2"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:504896"
     regulatory      1..296
                     /regulatory_class="promoter"
                     /note="PBAD"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     gene            318..891
                     /gene="gVIII"
                     /label=gVIII
     CDS             318..401
                     /codon_start=1
                     /gene="gVIII"
                     /product="major coat protein pVIII"
                     /label=gVIII
                     /protein_id="ACA60871.1"
                     /translation="MKKSLVLKASVAVATLVPMLSFAAEGEF"
     sig_peptide     318..398
                     /gene="gVIII"
     misc_feature    396..401
                     /gene="gVIII"
                     /label=unique EcoRI cloning site
                     /note="unique EcoRI cloning site"
     misc_feature    402..749
                     /gene="gVIII"
                     /note="spacer fragment; derived from lambda phage"
     misc_feature    750..755
                     /gene="gVIII"
                     /label=unique BglII cloning site
                     /note="unique BglII cloning site"
     CDS             751..891
                     /codon_start=1
                     /gene="gVIII"
                     /product="major coat protein pVIII"
                     /label=gVIII
                     /protein_id="ACA60872.1"
                     /translation="DLAKAAFDSLQASATEYIGYAWAMVVVIVGATIGIKLFKKFTSKA
                     S"
     primer_bind     complement(891..907)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    892..1074
                     /label=similar to beta-galactosidase
                     /note="similar to beta-galactosidase"
     rep_origin      1347..1803
                     /note="f1 phage; single strand DNA replication"
     rep_origin      1348..1861
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1925..2029
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2030..2890
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="confers ampicillin resistance"
                     /protein_id="ACA60873.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            2030..2890
                     /gene="bla"
                     /label=bla
     rep_origin      3061..3649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3459..3799
                     /label=ColE1 plasmid
                     /note="ColE1 plasmid"
     misc_feature    3835..3975
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(4239..5117)
                     /codon_start=1
                     /gene="araC"
                     /product="AraC"
                     /label=araC
                     /note="transcriptional regulator of PBAD promoter"
                     /protein_id="ACA60874.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     gene            complement(4239..5117)
                     /gene="araC"
                     /label=araC